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Mouse Anti-BRD2 Recombinant Antibody (CBYY-1864) (CBMAB-2213-YY)

This product is mouse antibody that recognizes BRD2. The antibody CBYY-1864 can be used for immunoassay techniques such as: IHC-P, IP, WB
See all BRD2 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-1864
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, E

Basic Information

Immunogen
Amino acids 214-233 within an internal region of BRD2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
bromodomain containing 2
Introduction
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Entrez Gene ID
6046
UniProt ID
P25440
Alternative Names
Bromodomain Containing 2; Really Interesting New Gene 3 Protein; BRD2 Intronic Transcript 1; O27.1.1; RING3; Female Sterile Homeotic-Related Gene 1; Bromodomain-Containing Protein 2; Bromodomain-Containing 2;
Function
May play a role in spermatogenesis or folliculogenesis (By similarity).
Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.
Biological Process
Neural tube closure Source: Ensembl
Nucleosome assembly Source: UniProtKB
Protein phosphorylation Source: FlyBase
Regulation of transcription by RNA polymerase II Source: UniProtKB
Spermatogenesis Source: ProtInc
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus. Detected on chromatin and nucleosomes.

Yu, Y., Fu, W., Xu, J., Lei, Y., Song, X., Liang, Z., ... & Li, C. (2021). Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis. Molecular Plant.

Jarończyk, K., Sosnowska, K., Zaborowski, A., Pupel, P., Bucholc, M., Małecka, E., ... & Archacki, R. (2021). Bromodomain-containing subunits BRD1, BRD2, and BRD13 are required for proper functioning of SWI/SNF complexes in Arabidopsis. Plant Communications, 100174.

Zhou, L., Han, Y. F., Yuan, C., & Duan, Z. Q. (2021). Screening and bioinformatics analysis of cellular proteins interacting with chicken bromodomain-containing protein 2 in DF-1 cells. British Poultry Science, (just-accepted).

Duan, Z., Han, Y., Zhou, L., Yuan, C., Wang, Y., Zhao, C., ... & Chen, J. (2020). Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Veterinary Research, 51(1), 1-18.

Wang, L. F., Wang, Y., Yang, Z. Y., Zhao, J., Sun, H. B., & Wu, S. L. (2020). Revealing binding selectivity of inhibitors toward bromodomain-containing proteins 2 and 4 using multiple short molecular dynamics simulations and free energy analyses. SAR and QSAR in Environmental Research, 31(5), 373-398.

Jiang, F., Wei, Q., Li, H., Li, H., Cui, Y., Ma, Y., ... & Chen, Y. (2020). Discovery of novel small molecule induced selective degradation of the bromodomain and extra-terminal (BET) bromodomain protein BRD4 and BRD2 with cellular potencies. Bioorganic & medicinal chemistry, 28(1), 115181.

Zong, J., Li, S., Wang, Y., Mo, W., Sun, R., & Yu, M. (2019). Bromodomain-containing protein 2 promotes lipolysis via ERK/HSL signalling pathway in white adipose tissue of mice. General and comparative endocrinology, 281, 105-116.

Hishiki, K., Akiyama, M., Kanegae, Y., Ozaki, K., Ohta, M., Tsuchitani, E., ... & Yamada, H. (2018). NF-κB signaling activation via increases in BRD2 and BRD4 confers resistance to the bromodomain inhibitor I-BET151 in U937 cells. Leukemia research, 74, 57-63.

Lee, D. J., Hwang, J. H., Ha, J., Yu, G. E., Kwon, S., Park, D. H., ... & Kim, C. W. (2018). Identification of a bromodomain-containing protein 2 (BRD2) gene polymorphic variant and its effects on pork quality traits in berkshire pigs. Korean journal for food science of animal resources, 38(4), 703.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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