Human BRD2 ELISA Kit (V2LY-0626-LY5527)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0075 ng/mL
Detection Range
0.015-3 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
88.1 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
bromodomain containing 2
Function
May play a role in spermatogenesis or folliculogenesis (By similarity).
Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly.
Biological Process
Neural tube closure Source: Ensembl
Nucleosome assembly Source: UniProtKB
Protein phosphorylation Source: FlyBase
Regulation of transcription by RNA polymerase II Source: UniProtKB
Spermatogenesis Source: ProtInc
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus. Detected on chromatin and nucleosomes.

Yu, Y., Fu, W., Xu, J., Lei, Y., Song, X., Liang, Z., ... & Li, C. (2021). Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis. Molecular Plant.

Jarończyk, K., Sosnowska, K., Zaborowski, A., Pupel, P., Bucholc, M., Małecka, E., ... & Archacki, R. (2021). Bromodomain-containing subunits BRD1, BRD2, and BRD13 are required for proper functioning of SWI/SNF complexes in Arabidopsis. Plant Communications, 100174.

Zhou, L., Han, Y. F., Yuan, C., & Duan, Z. Q. (2021). Screening and bioinformatics analysis of cellular proteins interacting with chicken bromodomain-containing protein 2 in DF-1 cells. British Poultry Science, (just-accepted).

Duan, Z., Han, Y., Zhou, L., Yuan, C., Wang, Y., Zhao, C., ... & Chen, J. (2020). Chicken bromodomain-containing protein 2 interacts with the Newcastle disease virus matrix protein and promotes viral replication. Veterinary Research, 51(1), 1-18.

Wang, L. F., Wang, Y., Yang, Z. Y., Zhao, J., Sun, H. B., & Wu, S. L. (2020). Revealing binding selectivity of inhibitors toward bromodomain-containing proteins 2 and 4 using multiple short molecular dynamics simulations and free energy analyses. SAR and QSAR in Environmental Research, 31(5), 373-398.

Jiang, F., Wei, Q., Li, H., Li, H., Cui, Y., Ma, Y., ... & Chen, Y. (2020). Discovery of novel small molecule induced selective degradation of the bromodomain and extra-terminal (BET) bromodomain protein BRD4 and BRD2 with cellular potencies. Bioorganic & medicinal chemistry, 28(1), 115181.

Zong, J., Li, S., Wang, Y., Mo, W., Sun, R., & Yu, M. (2019). Bromodomain-containing protein 2 promotes lipolysis via ERK/HSL signalling pathway in white adipose tissue of mice. General and comparative endocrinology, 281, 105-116.

Hishiki, K., Akiyama, M., Kanegae, Y., Ozaki, K., Ohta, M., Tsuchitani, E., ... & Yamada, H. (2018). NF-κB signaling activation via increases in BRD2 and BRD4 confers resistance to the bromodomain inhibitor I-BET151 in U937 cells. Leukemia research, 74, 57-63.

Lee, D. J., Hwang, J. H., Ha, J., Yu, G. E., Kwon, S., Park, D. H., ... & Kim, C. W. (2018). Identification of a bromodomain-containing protein 2 (BRD2) gene polymorphic variant and its effects on pork quality traits in berkshire pigs. Korean journal for food science of animal resources, 38(4), 703.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant BRD2, Active protein, His Tag (CAT#: V2LY-0526-LY2407)

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Isotype control

For research use only. Not intended for any clinical use.

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