Rabbit Anti-BUB1 Recombinant Antibody (
9C11) (V2LY-0725-LY1158)

Name: Rabbit Anti-BUB1 Recombinant Antibody (9C11)
SKU: V2LY-0725-LY1158
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-BUB1 Recombinant Antibody (9C11) (V2LY-0725-LY1158) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: Bub1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 116, 120 kDa
Observed band size: 130 kDa

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9C11

Application

ELISA, WB

Immunogen

A synthesized peptide derived from human Bub1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

BUB1 Mitotic Checkpoint Serine/Threonine Kinase

Entrez Gene ID

699

UniProt ID

O43683

Function

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Meiotic sister chromatid cohesion, centromeric Source: GO_Central
Mitotic cell cycle checkpoint Source: ProtInc
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Regulation of chromosome segregation Source: UniProtKB
Regulation of sister chromatid cohesion Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity).

PTM

Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.

Mamoor, S. (2021). Differential expression of BUB1 mitotic checkpoint serine/threonine kinase in cancers of the breast.

Mamoor, S. (2021). BUB1 is differentially expressed in lymph node metastasis in human breast cancer.

Huang, Z., Wang, S., Wei, H., Chen, H., Shen, R., Lin, R., ... & Lin, J. (2021). Inhibition of BUB1 suppresses tumorigenesis of osteosarcoma via blocking of PI3K/Akt and ERK pathways. Journal of Cellular and Molecular Medicine, 25(17), 8442-8453.

Long, Z., Wu, T., Tian, Q., Carlson, L. A., Wang, W., & Wu, G. (2021). Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma. Aging (Albany NY), 13(9), 12395.

Zhu, L. J., Pan, Y., Chen, X. Y., & Hou, P. F. (2020). BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncology letters, 19(5), 3506-3512.

Yu, H., Zhang, S., Ibrahim, A. N., Deng, Z., & Wang, M. (2019). Serine/threonine kinase BUB1 promotes proliferation and radio-resistance in glioblastoma. Pathology-Research and Practice, 215(8), 152508.

Piao, J., Zhu, L., Sun, J., Li, N., Dong, B., Yang, Y., & Chen, L. (2019). High expression of CDK1 and BUB1 predicts poor prognosis of pancreatic ductal adenocarcinoma. Gene, 701, 15-22.

Wang, L., Yang, X., An, N., & Liu, J. (2019). Bioinformatics analysis of BUB1 expression and gene regulation network in lung adenocarcinoma.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

BUB1 Matched Antibody Pair (150) (CAT#: APMAB-150LY)

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BUB1 Matched Antibody Pair (150) (CAT#: APMAB-150LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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