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BUB1

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Full Name
BUB1 Mitotic Checkpoint Serine/Threonine Kinase
Function
Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
Biological Process
Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Meiotic sister chromatid cohesion, centromeric Source: GO_Central
Mitotic cell cycle checkpoint Source: ProtInc
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Regulation of chromosome segregation Source: UniProtKB
Regulation of sister chromatid cohesion Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Nucleus; Kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity).
PTM
Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.

Anti-BUB1 antibodies

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Target: BUB1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: CBYY-2092
Application*: E, IF, WB
Target: BUB1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYY-1955
Application*: DB, WB
Target: BUB1
Host: Mouse
Specificity: Human, Mouse
Clone: CBYY-1871
Application*: F, IC, IF, IH, WB, IP
Target: BUB1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYY-0940
Application*: IF, IP, WB
Target: BUB1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Mouse, Rat, Human
Clone: CBYY-0939
Application*: WB, P
Target: BUB1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: CBYY-0938
Application*: WB, F
Target: BUB1
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYY-0937
Application*: E, IF, IP, WB
Target: BUB1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human
Clone: EG430
Application*: WB: 1:500~1:1000 ELISA: 1:40000
Target: BUB1
Host: Rabbit
Antibody Isotype: IgG
Specificity: Human, Mouse
Clone: EG429
Application*: IHC: 1:50~1:100 ELISA: 1:10000
Target: BUB1
Host: Mouse
Antibody Isotype: IgG1, κ
Specificity: Human
Clone: 2F9
Application*: WB, E
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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