Mouse Anti-BUB1 Recombinant Antibody (CBYY-2092) (CBMAB-2441-YY)

Name: Mouse Anti-BUB1 Recombinant Antibody (CBYY-2092)
SKU: CBMAB-2441-YY
Rating: 5 (1 reviews)

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Published Data

CBMAB-2441-YY in WB

The interaction of MAD2 with MAD1, and with BUBR1 in tumors as explored by Co-IP. ns, no significant. * P<0.05, ** P<0.01. ...View More

Song, J., Chen, Y., Yu, H., Zheng, L., Wang, Y., & Li, D. (2023). Proline-rich acidic protein 1 upregulates mitotic arrest deficient 1 to promote cisplatin-resistance of colorectal carcinoma by restraining mitotic checkpoint complex assembly. Journal of Cancer, 14(9), 1515.

CBMAB-2441-YY in WB

Confirmation of BUB1B and CIT knockdown by quantitative RT-PCR and immunoblotting after 72 h of siRNA (si) transfection. mRNA levels were normalized to GAPDH. Consistent immunoblotting results were obtained from two experiments. ...View More

Cheng, C. K., Yung, Y. L., Chan, H. Y., Leung, K. T., Chan, K. Y., Leung, A. W., ... & Ng, M. H. (2023). Deep genomic characterization highlights complexities and prognostic markers of pediatric acute myeloid leukemia. Communications Biology, 6(1), 356.

CBMAB-2441-YY in Immunoblot

Left panel: immunoblotting against APC2 and BubR1, an MCC component, shows equal loading of all reactants for the reconstitution experiments, P: phosphorylated APC/C, SP: phosphorylated SUMOylated APC/C, -: sample without APC/C (i.e. MCC-Cdc20 alone). Right panel: immunoblotting against APC2 shows that equal amounts of APC/C are present in peak fractions, whereas immunoblotting against MCC components, BubR1, Bub3 and Mad2 shows reduced amount of MCC in the SUMOylated APC/C sample. ...View More

Yatskevich, S., Kroonen, J. S., Alfieri, C., Tischer, T., Howes, A. C., Clijsters, L., ... & Barford, D. (2021). Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell reports, 34(13).

CBMAB-2441-YY in WB

WB analysis for MAD2L1 (MAD2) and BUB1B (BUBR1) in the 3 GC cell lines cultured in adherence (ADH), as sphere (CSCs) or re-differentiated cells (DIF). ...View More

Pajuelo-Lozano, N., Alcalá, S., Sainz Jr, B., Perona, R., & Sanchez-Perez, I. (2020). Targeting MAD2 modulates stemness and tumorigenesis in human Gastric Cancer cell lines. Theranostics, 10(21), 9601.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-2092

Application

E, IF, IP, WB, P

Immunogen

Recombinant protein corresponding to amino acids 1-350 of BUBR1 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

BUB1 Mitotic Checkpoint Serine/Threonine Kinase

Introduction

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forMouse of cancer. Alternate splicing results in multiple transcript variants.

Entrez Gene ID

699

UniProt ID

O43683

Alternative Names

BUB1 Mitotic Checkpoint Serine/Threonine Kinase; BUB1L; BUB1A; HBUB1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog); Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast); Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1;

Function

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

Biological Process

Apoptotic process Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Meiotic sister chromatid cohesion, centromeric Source: GO_Central
Mitotic cell cycle checkpoint Source: ProtInc
Mitotic spindle assembly checkpoint Source: GO_Central
Mitotic spindle organization Source: Reactome
Regulation of chromosome segregation Source: UniProtKB
Regulation of sister chromatid cohesion Source: UniProtKB
Viral process Source: UniProtKB-KW

Cellular Location

Nucleus; Kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, KNL1 and INCENP are required for kinetochore localization (By similarity).

PTM

Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.

Mamoor, S. (2021). Differential expression of BUB1 mitotic checkpoint serine/threonine kinase in cancers of the breast.

Mamoor, S. (2021). BUB1 is differentially expressed in lymph node metastasis in human breast cancer.

Huang, Z., Wang, S., Wei, H., Chen, H., Shen, R., Lin, R., ... & Lin, J. (2021). Inhibition of BUB1 suppresses tumorigenesis of osteosarcoma via blocking of PI3K/Akt and ERK pathways. Journal of Cellular and Molecular Medicine, 25(17), 8442-8453.

Long, Z., Wu, T., Tian, Q., Carlson, L. A., Wang, W., & Wu, G. (2021). Expression and prognosis analyses of BUB1, BUB1B and BUB3 in human sarcoma. Aging (Albany NY), 13(9), 12395.

Zhu, L. J., Pan, Y., Chen, X. Y., & Hou, P. F. (2020). BUB1 promotes proliferation of liver cancer cells by activating SMAD2 phosphorylation. Oncology letters, 19(5), 3506-3512.

Yu, H., Zhang, S., Ibrahim, A. N., Deng, Z., & Wang, M. (2019). Serine/threonine kinase BUB1 promotes proliferation and radio-resistance in glioblastoma. Pathology-Research and Practice, 215(8), 152508.

Piao, J., Zhu, L., Sun, J., Li, N., Dong, B., Yang, Y., & Chen, L. (2019). High expression of CDK1 and BUB1 predicts poor prognosis of pancreatic ductal adenocarcinoma. Gene, 701, 15-22.

Wang, L., Yang, X., An, N., & Liu, J. (2019). Bioinformatics analysis of BUB1 expression and gene regulation network in lung adenocarcinoma.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

BUB1 Matched Antibody Pair (150) (CAT#: APMAB-150LY)

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BUB1 Matched Antibody Pair (150) (CAT#: APMAB-150LY)

Isotype control

For research use only. Not intended for any clinical use.

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