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Mouse Anti-CA1 Recombinant Antibody (CBFYC-0681) (CBMAB-C0735-FY)

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Published Data
CBMAB-C0735-FY-1
Mouse Anti-CA1 Recombinant Antibody (CBFYC-0681) (CBMAB-C0735-FY) in IHC
IHC for CA 1 in normal samples (under 20× magnification). ...View More
Venugopal, D. C., Ravindran, S., Shyamsundar, V., Sankarapandian, S., Krishnamurthy, A., Sivagnanam, A., ... & Ramshankar, V. (2022). Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis-An Indian Study. Journal of Personalized Medicine, 12(2), 208.
CBMAB-C0735-FY-2
Mouse Anti-CA1 Recombinant Antibody (CBFYC-0681) (CBMAB-C0735-FY) in IHC
IHC of CA 1 in OSMF samples (under 20× magnification). ...View More
Venugopal, D. C., Ravindran, S., Shyamsundar, V., Sankarapandian, S., Krishnamurthy, A., Sivagnanam, A., ... & Ramshankar, V. (2022). Integrated Proteomics Based on 2D Gel Electrophoresis and Mass Spectrometry with Validations: Identification of a Biomarker Compendium for Oral Submucous Fibrosis-An Indian Study. Journal of Personalized Medicine, 12(2), 208.
Datasheet
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYC-0681
Application
E, WB, P, IF, IP
Immunogen
Amino acids 45-64 near the N-terminus of CA I (carbonic anhydrase) of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Dog, Pig
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-261
More Infomation

Target

Full Name
carbonic anhydrase 1
Introduction
This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8.
Entrez Gene ID
759
UniProt ID
P00915
Alternative Names
CAB; CA-I; Car1; HEL-S-11
Function
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
Biological Process
Bicarbonate transport Source: Reactome
Interleukin-12-mediated signaling pathway Source: Reactome
One-carbon metabolic process Source: GO_Central
Cellular Location
Cytoplasm

Feng, Y., Zhong, M., Tang, Y., Liu, X., Liu, Y., Wang, L., & Zhou, H. (2020). The role and underlying mechanism of exosomal CA1 in chemotherapy resistance in diffuse large B cell lymphoma. Molecular Therapy-Nucleic Acids, 21, 452-463.

Yuan, L., Wang, M., Liu, T., Lei, Y., Miao, Q., Li, Q., ... & Chang, X. (2019). Carbonic anhydrase 1-mediated calcification is associated with atherosclerosis, and methazolamide alleviates its pathogenesis. Frontiers in pharmacology, 10, 766.

Lu, D., Peng, X., Jia, X., Li, G., Tan, N., Wei, Z., ... & Liu, J. (2019). Carbonic Anhydrase I modifies SOD1-induced motor neuron toxicity in< em> Drosophila via ER stress pathway. Journal of Neuroscience and Neurological Disorders, 3, 135-144.

Kardoush, M. I., Ward, B. J., & Ndao, M. (2017). Serum carbonic anhydrase 1 is a biomarker for diagnosis of human schistosoma mansoni infection. The American journal of tropical medicine and hygiene, 96(4), 842.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CA1 Matched Antibody Pair (162) (CAT#: APMAB-162LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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