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Mouse Anti-CA1 Recombinant Antibody (D4) (CBMAB-A1009-LY)

The product is antibody recognizes CA1. The antibody M1 immunoassay techniques such as: WB, ELISA.
See all CA1 antibodies

Summary

Host Animal
Mouse
Specificity
Mouse, Rat
Clone
D4
Antibody Isotype
IgG1, κ
Application
E, WB, IF, IP

Basic Information

Immunogen
N-terminus of CA-I (carbonic anhydrase) of mouse origin.
Host Species
Mouse
Specificity
Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
0.09% sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
carbonic anhydrase 1
Introduction
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq]
Entrez Gene ID
759
UniProt ID
P00915
Alternative Names
Car1
Function
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea.
Biological Process
Bicarbonate transport Source: Reactome
Interleukin-12-mediated signaling pathway Source: Reactome
One-carbon metabolic process Source: GO_Central
Cellular Location
Cytoplasm
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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