Rabbit Anti-CARS Recombinant Antibody (
8B11) (V2LY-0725-LY974)

Name: Rabbit Anti-CARS Recombinant Antibody (8B11)
SKU: V2LY-0725-LY974
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CARS Recombinant Antibody (8B11) (V2LY-0725-LY974) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate
All lanes: CARS antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 86, 83, 95 kDa
Observed band size: 95 kDa

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

8B11

Application

ELISA, WB

Immunogen

A synthesized peptide derived from human CARS.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cysteinyl-TRNA Synthetase

Entrez Gene ID

833

UniProt ID

P49589

Function

Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).

Biological Process

Cysteinyl-tRNA aminoacylation Source: UniProtKB
tRNA aminoacylation for protein translation Source: Reactome

Cellular Location

Cytoplasm

Involvement in disease

Microcephaly, developmental delay, and brittle hair syndrome (MDBH): An autosomal recessive disorder characterized by developmental delay, motor and cognitive disabilities, brittle hair and nails, failure to thrive, and short stature.

Meng, Q., Li, C. X., & Long, D. (2021). CARS as a Prognosis Factor in Clear Cell Renal Cell Carcinoma and Its Association with Tumor Immunity. International Journal of General Medicine, 14, 3197.

Yuan, M., Yan, R., Zhang, Y., Qiu, Y., Jiang, Z., Liu, H., ... & Gao, P. (2021). CARS senses cysteine deprivation to activate AMPK for cell survival. The EMBO Journal, 40(21), e108028.

Kuo, M. E., Theil, A. F., Kievit, A., Malicdan, M. C., Introne, W. J., Christian, T., ... & Mancini, G. M. (2019). Cysteinyl-tRNA synthetase mutations cause a multi-system, recessive disease that includes microcephaly, developmental delay, and brittle hair and nails. The American Journal of Human Genetics, 104(3), 520-529.

Nishimura, A., Nasuno, R., Yoshikawa, Y., Jung, M., Ida, T., Matsunaga, T., ... & Akaike, T. (2019). Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells. Journal of Biological Chemistry, 294(37), 13781-13788.

Akaike, T., Motohashi, H., Fukuto, J., & Nagy, P. (2018). Cysteinyl-tRNA synthetase (CARS) controls endogenous hydropersulfide production and mitochondrial respiration. Free Radical Biology and Medicine, 120, S21.

Akaike, T., Ida, T., Wei, F. Y., Nishida, M., Kumagai, Y., Alam, M. M., ... & Motohashi, H. (2017). Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Nature communications, 8(1), 1-15.

Ida, T., Nishimura, A., Morita, M., Motohashi, H., & Akaike, T. (2017). Cysteine Hydropersulfide Production Catalyzed by Cysteinyl-tRNA Synthetases. Free Radical Biology and Medicine, 112, 189-190.

Akaike, T., Nishimura, A., Ida, T., Matsunaga, T., Morita, M., & Motohashi, H. (2017). Moonlighting Functions of Cysteinyl-tRNA Synthetases: Cycteine Hydropersulfide Production and Regulation of Mitochondrial Biogenesis and Bioenergetics. Free Radical Biology and Medicine, 112, 182-183.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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