Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Rabbit Anti-CARS Recombinant Antibody (CBXC-1879) (CBMAB-C5086-CQ)


See all CARS antibodies
Online Inquiry

Summary

Host Animal
Rabbit
Specificity
Human, Rat
Clone
CBXC-1879
Antibody Isotype
IgG
Application
WB, ELISA, IHC-P

Basic Information

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 650-748 of human CARS.
Host Species
Rabbit
Specificity
Human, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:5,000
IHC-P1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.3, 50% glycerol, 0.05% BSA
Preservative
0.05% sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cysteinyl-TRNA Synthetase
Introduction
This gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants.
Entrez Gene ID
833
UniProt ID
P49589
Alternative Names
Cysteinyl-TRNA Synthetase; Cysteine TRNA Ligase 1, Cytoplasmic; EC 6.1.1.16; CYSRS; Cysteine--TRNA Ligase, Cytoplasmic; Cysteine Translase; MGC:11246; EC 6.1.1; CARS1;
Function
Catalyzes the ATP-dependent ligation of cysteine to tRNA(Cys).
Biological Process
Cysteinyl-tRNA aminoacylation Source: UniProtKB
tRNA aminoacylation for protein translation Source: Reactome
Cellular Location
Cytoplasm
Involvement in disease
Microcephaly, developmental delay, and brittle hair syndrome (MDBH): An autosomal recessive disorder characterized by developmental delay, motor and cognitive disabilities, brittle hair and nails, failure to thrive, and short stature.
More Infomation

Meng, Q., Li, C. X., & Long, D. (2021). CARS as a Prognosis Factor in Clear Cell Renal Cell Carcinoma and Its Association with Tumor Immunity. International Journal of General Medicine, 14, 3197.

Yuan, M., Yan, R., Zhang, Y., Qiu, Y., Jiang, Z., Liu, H., ... & Gao, P. (2021). CARS senses cysteine deprivation to activate AMPK for cell survival. The EMBO Journal, 40(21), e108028.

Kuo, M. E., Theil, A. F., Kievit, A., Malicdan, M. C., Introne, W. J., Christian, T., ... & Mancini, G. M. (2019). Cysteinyl-tRNA synthetase mutations cause a multi-system, recessive disease that includes microcephaly, developmental delay, and brittle hair and nails. The American Journal of Human Genetics, 104(3), 520-529.

Nishimura, A., Nasuno, R., Yoshikawa, Y., Jung, M., Ida, T., Matsunaga, T., ... & Akaike, T. (2019). Mitochondrial cysteinyl-tRNA synthetase is expressed via alternative transcriptional initiation regulated by energy metabolism in yeast cells. Journal of Biological Chemistry, 294(37), 13781-13788.

Akaike, T., Motohashi, H., Fukuto, J., & Nagy, P. (2018). Cysteinyl-tRNA synthetase (CARS) controls endogenous hydropersulfide production and mitochondrial respiration. Free Radical Biology and Medicine, 120, S21.

Akaike, T., Ida, T., Wei, F. Y., Nishida, M., Kumagai, Y., Alam, M. M., ... & Motohashi, H. (2017). Cysteinyl-tRNA synthetase governs cysteine polysulfidation and mitochondrial bioenergetics. Nature communications, 8(1), 1-15.

Ida, T., Nishimura, A., Morita, M., Motohashi, H., & Akaike, T. (2017). Cysteine Hydropersulfide Production Catalyzed by Cysteinyl-tRNA Synthetases. Free Radical Biology and Medicine, 112, 189-190.

Akaike, T., Nishimura, A., Ida, T., Matsunaga, T., Morita, M., & Motohashi, H. (2017). Moonlighting Functions of Cysteinyl-tRNA Synthetases: Cycteine Hydropersulfide Production and Regulation of Mitochondrial Biogenesis and Bioenergetics. Free Radical Biology and Medicine, 112, 182-183.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-CARS Recombinant Antibody (CBXC-1879)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry