Rabbit Anti-CASP2 Recombinant Antibody (
3D4) (V2LY-0725-LY1353)

Name: Rabbit Anti-CASP2 Recombinant Antibody (3D4)
SKU: V2LY-0725-LY1353
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-CASP2 Recombinant Antibody (3D4) (V2LY-0725-LY1353) in WB

Western Blot
Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate
All lanes: Caspase-2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 51, 35, 11 kDa
Observed band size: 51 kDa

Rabbit Anti-CASP2 Recombinant Antibody (3D4) (V2LY-0725-LY1353) in IHC

IHC image of V2LY-0725-LY1353 diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-CASP2 Recombinant Antibody (3D4) (V2LY-0725-LY1353) in IHC

IHC image of V2LY-0725-LY1353 diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

3D4

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human Caspase-2.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Caspase 2

Entrez Gene ID

835

UniProt ID

P42575

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321).
Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Aging Source: Ensembl
Apoptotic process Source: GO_Central
Apoptotic signaling pathway Source: UniProtKB
Brain development Source: Ensembl
Cellular response to mechanical stimulus Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: UniProtKB
Ectopic germ cell programmed cell death Source: Ensembl
Execution phase of apoptosis Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Luteolysis Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Neural retina development Source: Ensembl
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of neuron apoptotic process Source: Ensembl
Protein processing Source: BHF-UCL
Regulation of apoptotic process Source: Reactome

Cellular Location

Cytosol; Mitochondrion; Nucleus; Cytoplasm; Membrane

PTM

The mature protease can process its own propeptide, but not that of other caspases.

Kopeina, G. S., & Zhivotovsky, B. (2021). Caspase-2 as a master regulator of genomic stability. Trends in Cell Biology.

Lim, Y., Dorstyn, L., & Kumar, S. (2021). The p53-caspase-2 axis in the cell cycle and DNA damage response. Experimental & Molecular Medicine, 53(4), 517-527.

Ochiai, A., Sawaguchi, S., Memezawa, S., Seki, Y., Morimoto, T., Oizumi, H., ... & Yamauchi, J. (2021). Knockdown of Golgi Stress-Responsive Caspase-2 Ameliorates HLD17-Associated AIMP2 Mutant-Mediated Inhibition of Oligodendroglial Cell Morphological Differentiation. Neurochemical Research, 1-15.

Yang, L., Dou, Y., Sui, Z., Cheng, H., Liu, X., Wang, Q., ... & Xu, M. (2020). Upregulated miRNA‑182‑5p expression in tumor tissue and peripheral blood samples from patients with non‑small cell lung cancer is associated with downregulated Caspase 2 expression. Experimental and therapeutic medicine, 19(1), 603-610.

Petroni, G., Vitale, I., & Galluzzi, L. (2020). Caspase 2 and p53 Reunited in Tumor Control. Trends in Cell Biology, 30(12), 917-918.

Smith, B. R., Nelson, K. M., Kemper, L. J., Leinonen-Wright, K., Petersen, A., Keene, C. D., & Ashe, K. H. (2019). A soluble tau fragment generated by caspase-2 is associated with dementia in Lewy body disease. Acta neuropathologica communications, 7(1), 1-9.

Vigneswara, V., & Ahmed, Z. (2019). Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2. Cell death & disease, 10(2), 1-17.

Liu, P., Smith, B. R., Huang, E. S., Mahesh, A., Vonsattel, J. P. G., Petersen, A. J., ... & Ashe, K. H. (2019). A soluble truncated tau species related to cognitive dysfunction and caspase-2 is elevated in the brain of Huntington’s disease patients. Acta neuropathologica communications, 7(1), 1-13.

Vitale, I., Manic, G., Castedo, M., & Kroemer, G. (2017). Caspase 2 in mitotic catastrophe: The terminator of aneuploid and tetraploid cells. Molecular & cellular oncology, 4(3), e1299274.

Forsberg, J., Zhivotovsky, B., & Olsson, M. (2017). Caspase-2: an orphan enzyme out of the shadows. Oncogene, 36(39), 5441-5444.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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