Mouse Anti-CASP2 Recombinant Antibody (CBFYC-0376) (CBMAB-C0416-FY)

Name: Mouse Anti-CASP2 Recombinant Antibody (CBFYC-0376)
SKU: CBMAB-C0416-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CASP2 Recombinant Antibody (CBFYC-0376) (CBMAB-C0416-FY) in WB

High Pi treatment reduces alternative splicing of Caspase-2 but has no effect on Caspase-9 and BCL-x splicing in HEK293 cells. Actin was used as a loading control. ...View More

Hetz, R., Beeler, E., Janoczkin, A., Kiers, S., Li, L., Willard, B. B., ... & He, P. (2022). Excessive inorganic phosphate burden perturbed intracellular signaling: quantitative proteomics and phosphoproteomics analyses. Frontiers in Nutrition, 8, 765391.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-0376

Application

ELISA, IF, WB, IP

Immunogen

Amino acids 153-297 of caspase-2S of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 32-169

More Infomation

Target

Full Name

Caspase 2

Introduction

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of this gene may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Entrez Gene ID

835

UniProt ID

P42575

Alternative Names

Caspase 2; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 2; Protein Phosphatase 1, Regulatory Subunit 57; Protease ICH-1; EC 3.4.22.55; CASP-2; NEDD-2; NEDD2

Function

Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival (PubMed:15073321).
Associates with PIDD1 and CRADD to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis in response to genotoxic stress (PubMed:15073321).

Biological Process

Activation of cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
Aging Source: Ensembl
Apoptotic process Source: GO_Central
Apoptotic signaling pathway Source: UniProtKB
Brain development Source: Ensembl
Cellular response to mechanical stimulus Source: UniProtKB
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest Source: UniProtKB
Ectopic germ cell programmed cell death Source: Ensembl
Execution phase of apoptosis Source: UniProtKB
Extrinsic apoptotic signaling pathway in absence of ligand Source: GO_Central
Intrinsic apoptotic signaling pathway in response to DNA damage Source: GO_Central
Luteolysis Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Neural retina development Source: Ensembl
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of neuron apoptotic process Source: Ensembl
Protein processing Source: BHF-UCL
Regulation of apoptotic process Source: Reactome

Cellular Location

Cytosol; Mitochondrion; Nucleus; Cytoplasm; Membrane

PTM

The mature protease can process its own propeptide, but not that of other caspases.

Kopeina, G. S., & Zhivotovsky, B. (2021). Caspase-2 as a master regulator of genomic stability. Trends in Cell Biology.

Lim, Y., Dorstyn, L., & Kumar, S. (2021). The p53-caspase-2 axis in the cell cycle and DNA damage response. Experimental & Molecular Medicine, 53(4), 517-527.

Ochiai, A., Sawaguchi, S., Memezawa, S., Seki, Y., Morimoto, T., Oizumi, H., ... & Yamauchi, J. (2021). Knockdown of Golgi Stress-Responsive Caspase-2 Ameliorates HLD17-Associated AIMP2 Mutant-Mediated Inhibition of Oligodendroglial Cell Morphological Differentiation. Neurochemical Research, 1-15.

Yang, L., Dou, Y., Sui, Z., Cheng, H., Liu, X., Wang, Q., ... & Xu, M. (2020). Upregulated miRNA‑182‑5p expression in tumor tissue and peripheral blood samples from patients with non‑small cell lung cancer is associated with downregulated Caspase 2 expression. Experimental and therapeutic medicine, 19(1), 603-610.

Petroni, G., Vitale, I., & Galluzzi, L. (2020). Caspase 2 and p53 Reunited in Tumor Control. Trends in Cell Biology, 30(12), 917-918.

Smith, B. R., Nelson, K. M., Kemper, L. J., Leinonen-Wright, K., Petersen, A., Keene, C. D., & Ashe, K. H. (2019). A soluble tau fragment generated by caspase-2 is associated with dementia in Lewy body disease. Acta neuropathologica communications, 7(1), 1-9.

Vigneswara, V., & Ahmed, Z. (2019). Pigment epithelium-derived factor mediates retinal ganglion cell neuroprotection by suppression of caspase-2. Cell death & disease, 10(2), 1-17.

Liu, P., Smith, B. R., Huang, E. S., Mahesh, A., Vonsattel, J. P. G., Petersen, A. J., ... & Ashe, K. H. (2019). A soluble truncated tau species related to cognitive dysfunction and caspase-2 is elevated in the brain of Huntington’s disease patients. Acta neuropathologica communications, 7(1), 1-13.

Vitale, I., Manic, G., Castedo, M., & Kroemer, G. (2017). Caspase 2 in mitotic catastrophe: The terminator of aneuploid and tetraploid cells. Molecular & cellular oncology, 4(3), e1299274.

Forsberg, J., Zhivotovsky, B., & Olsson, M. (2017). Caspase-2: an orphan enzyme out of the shadows. Oncogene, 36(39), 5441-5444.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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