Mouse Anti-CCL28 Recombinant Antibody (CBYY-C1085) (CBMAB-C2522-YY)

Name: Mouse Anti-CCL28 Recombinant Antibody (CBYY-C1085)
SKU: CBMAB-C2522-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CCL28 Recombinant Antibody (CBYY-C1085) (CBMAB-C2522-YY) in WB

WB data of CCL28 and proteins involved in WNT signaling (n = 3 independent samples/group). ...View More

Sun, L., Zhang, Y., Cai, J., Rimal, B., Rocha, E. R., Coleman, J. P., ... & Gonzalez, F. J. (2023). Bile salt hydrolase in non-enterotoxigenic Bacteroides potentiates colorectal cancer. Nature communications, 14(1), 755.

Mouse Anti-CCL28 Recombinant Antibody (CBYY-C1085) (CBMAB-C2522-YY) in WB

Mice were pre-injected with control antibody (Ctrl Ab) or neutralizing antibodies against IL-1β (anti-IL-1β) and/or TNF-α (anti-TNF-α) into the intraspinal cord for 12 hrs, and then subjected to sham or SCI surgery. After another 12 hrs, the spinal cord samples were analyzed by Western blotting for detecting CCL28 protein level. ...View More

Wang, P., Qi, X., Xu, G., Liu, J., Guo, J., Li, X., ... & Sun, H. (2019). CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells. Aging (Albany NY), 11(18), 7402.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C1085

Application

IF, WB, ELISA, IP

Immunogen

Amino acids 103-130 at the C-terminus of CCL28 of mouse origin.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

chemokine (C-C motif) ligand 28

Entrez Gene ID

56477

UniProt ID

Q9NRJ3

Alternative Names

Cyclin D1; B-Cell Lymphoma 1 Protein; B-Cell CLL/Lymphoma 1; BCL-1 Oncogene; PRAD1 Oncogene; PRAD1; BCL1; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1);

Function

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.

Biological Process

Antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
Cell chemotaxis Source: UniProtKB
Chemotaxis Source: UniProtKB
Cytolysis in other organism Source: UniProtKB
G protein-coupled receptor signaling pathway Source: Reactome
Immune response Source: InterPro
Killing of cells of other organism Source: UniProtKB
Negative regulation of leukocyte tethering or rolling Source: UniProtKB
Positive regulation of cell-matrix adhesion Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: Ensembl
Response to nutrient Source: Ensembl

Cellular Location

Secreted

Iwamoto, R., Takahashi, T., Yoshimi, K., Imai, Y., Koide, T., Hara, M., ... & Yukita, A. (2021). Chemokine ligand 28 (CCL28) negatively regulates trabecular bone mass by suppressing osteoblast and osteoclast activities. Journal of Bone and Mineral Metabolism, 1-14.

Liu, B., & Wei, C. (2021). Hypoxia Induces Overexpression of CCL28 to Recruit Treg Cells to Enhance Angiogenesis in Lung Adenocarcinoma. Journal of Environmental Pathology, Toxicology and Oncology, 40(1).

Noor, S., Qousain, S. T., & Muhammad, S. A. (2019). In silico docking analysis of CCL28 (CC motif chemokine ligand 28) and astragalin as the potential inhibitor of rheumatoid arthritis. F1000Research, 8(313), 313.

Wang, P., Qi, X., Xu, G., Liu, J., Guo, J., Li, X., ... & Sun, H. (2019). CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells. Aging (Albany NY), 11(18), 7402.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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