Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY)

Name: Mouse Anti-CD276 Recombinant Antibody (CBYY-0973)
SKU: CBMAB-0976-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in Immunoblot

Immunoblots of B7-H3 treated with or without PNGase F at 37 °C for 3 h. ...View More

Wang, M., Zhang, Z., Chen, M., Lv, Y., Tian, S., Meng, F., ... & Wang, W. (2023). FDW028, a novel FUT8 inhibitor, impels lysosomal proteolysis of B7-H3 via chaperone-mediated autophagy pathway and exhibits potent efficacy against metastatic colorectal cancer. Cell Death & Disease, 14(8), 495. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in Immunoblot and IP

Co-IP assays showed the effects of FUT8 knockdown on the interaction between B7-H3 and HSC70 or LAMP2A. ...View More

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in IHC

Immunohistochemical staining for B7-H3, PD-L1, and CTLA-4. B7-H3 positive staining (A); B7-H3 negative staining (B); PD-L1 positive staining (C); CTLA-4 positive staining (D). ...View More

Lee, J. H., Kim, Y. J., Ryu, H. W., Shin, S. W., Kim, E. J., Shin, S. H., ... & Lee, H. J. (2023). B7-H3 expression is associated with high PD-L1 expression in clear cell renal cell carcinoma and predicts poor prognosis. Diagnostic pathology, 18(1), 36. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in IHC

Renal cell carcinoma (RCC) cells stained with hematoxylin and eosin (H&E). Clear cell RCC (ccRCC) (A); immune cells with ccRCC (B); necrosis with a sarcomatoid pattern (C); .B7-H3 expression in sarcomatoid pattern (D). ...View More

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in IHC

Representative images of IHC staining of B7H3 expression in B7H3-WT re-expressed 4T1-B7H3KO xenograft tumor sections after treatment (left, n = 5 mice per group). HPF, ×400 magnification. Quantitative IHC analysis of B7H3 (right). ...View More

Huang, Y., Zhang, H. L., Li, Z. L., Du, T., Chen, Y. H., Wang, Y., ... & Deng, R. (2021). FUT8-mediated aberrant N-glycosylation of B7H3 suppresses the immune response in triple-negative breast cancer. Nature communications, 12(1), 2672. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD276 Recombinant Antibody (CBYY-0973) (CBMAB-0976-YY) in IHC

Fluorescence images showing immunostaining for CD276 (red). ...View More

Smith, T. A., Ghergherehchi, C. L., Tucker, H. O., & Bittner, G. D. (2020). Coding transcriptome analyses reveal altered functions underlying immunotolerance of PEG-fused rat sciatic nerve allografts. Journal of Neuroinflammation, 17, 1-24. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0973

Application

WB, IP, ELISA, IF, IHC-P

Immunogen

Amino acids 166-465 mapping within an N-terminal extracellular domain of B7-H3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

Center

More Infomation

Target

Full Name

CD276 Molecule

Introduction

CD276 (CD276 Molecule) is a Protein Coding gene.
Diseases associated with CD276 include Neuroblastoma and Acute Cervicitis.
Among its related pathways are T Cell Co-Signaling Pathway: Ligand-Receptor Interactions and NF-kappaB Signaling.
Gene Ontology (GO) annotations related to this gene include receptor binding.
An important paralog of this gene is LOC102723996.

Entrez Gene ID

80381

UniProt ID

Q5ZPR3

Alternative Names

CD276 Molecule; CD276 Antigen; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7-H3; B7H3; B7RP-2;

Function

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Biological Process

Positive regulation of interferon-gamma production Source: HGNC-UCL
Positive regulation of T cell proliferation Source: HGNC-UCL
Regulation of cytokine production Source: GO_Central
Regulation of immune response Source: HGNC-UCL
T cell activation Source: UniProtKB
T cell receptor signaling pathway Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 29-466
Helical: 467-487
Cytoplasmic: 488-534

Fu, M., Zhang, J., Li, W., He, S., Zhang, J., Tennant, D., ... & Mao, Y. (2021). Gene Clusters Based on OLIG2 and CD276 Could Distinguish Molecular Profiling in Glioblastoma.

Wang, M. Y., Qi, B., Wang, F., Lin, Z. R., Li, M. Y., Yin, W. J., ... & Chen, D. P. (2021). PBK phosphorylates MSL1 to elicit epigenetic modulation of CD276 in nasopharyngeal carcinoma. Oncogenesis, 10(1), 1-14.

Liu, X., Zhelev, D., Adams, C., Chen, C., Mellors, J. W., & Dimitrov, D. S. (2021). Effective killing of cells expressing CD276 (B7-H3) by a bispecific T cell engager based on a new fully human antibody. Translational oncology, 14(12), 101232.

Kanchan, R. K., Perumal, N., Atri, P., Chirravuri Venkata, R., Thapa, I., Klinkebiel, D. L., ... & Mahapatra, S. (2020). MiR‐1253 exerts tumor‐suppressive effects in medulloblastoma via inhibition of CDK6 and CD276 (B7‐H3). Brain Pathology, 30(4), 732-745.

Li, R., Zatloukalova, P., Muller, P., Gil-Mir, M., Kote, S., Wilkinson, S., ... & Vojtesek, B. (2020). The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276. Cellular & molecular biology letters, 25(1), 1-21.

Cheng, R., Wang, B., Cai, X. R., Chen, Z. S., Du, Q., Zhou, L. Y., ... & Chen, Y. L. (2020). CD276 promotes vasculogenic mimicry formation in hepatocellular carcinoma via the PI3K/AKT/MMPs pathway. OncoTargets and therapy, 13, 11485.

Yu, X., Wei, B., Su, R., Yao, J., Feng, X., Jiang, G., ... & Zhou, L. (2019). A risk assessment model of acute liver allograft rejection by genetic polymorphism of CD 276. Molecular genetics & genomic medicine, 7(6), e689.

Son, Y., Kwon, S. M., & Cho, J. Y. (2019). CD276 (B7‐H3) Maintains Proliferation and Regulates Differentiation in Angiogenic Function in Late Endothelial Progenitor Cells. Stem Cells, 37(3), 382-394.

Bao, R., Wang, Y., Lai, J., Zhu, H., Zhao, Y., Li, S., ... & Liu, Z. (2018). Enhancing anti-PD-1/PD-L1 immune checkpoint inhibitory cancer therapy by CD276-targeted photodynamic ablation of tumor cells and tumor vasculature. Molecular pharmaceutics, 16(1), 339-348.

Hwang, J. Y., Jeong, J. M., Kwon, M. G., Seo, J. S., Hwang, S. D., Son, M. H., ... & Park, C. I. (2018). Olive flounder CD276 (B7-H3) a coinhibitory molecule for T cells: Responses during viral hemorrhagic septicemia virus (VHSV) stimulation. Fish & shellfish immunology, 73, 228-233.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CD276 Matched Antibody Pair (215) (CAT#: APMAB-215LY)

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Isotype control

For research use only. Not intended for any clinical use.

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