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Mouse Anti-CD320 Recombinant Antibody (4F2) (CBMAB-C6922-LY)

This product is antibody recognizes CD320. The antibody 4F2 immunoassay techniques such as: ELISA, WB.
See all CD320 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F2
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
CD320 (AAH00668, 1 a.a. ~ 282 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.Immunogen sequence: MSGGWMAQVG AWRTGALGLA LLLLLGLGLG LEAAASPLST PTSAQAAGPS SGSCPPTKFQ CRTSGLCVPL TWRCDRDLDC SDGSDEEECR IEPCTQKGQC PPPPGLPCPC
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
CD320 molecule
Introduction
CD320 (CD320 Molecule) is a Protein Coding gene. Diseases associated with CD320 include Methylmalonic Aciduria, Transient, Due To Transcobalamin Receptor Defect and Methylmalonic Acidemia Due To Transcobalamin Receptor Defect. Among its related pathways are Diseases of metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include growth factor activity and cobalamin binding.
Entrez Gene ID
51293
UniProt ID
Q9NPF0
Alternative Names
CD320 Molecule; CD320 Antigen; 8D6 Antigen; FDC-Signaling Molecule 8D6; Transcobalamin Receptor; FDC-SM-8D6; TCBLR; 8D6A; 8D6;
Function
Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389).
Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213).
Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470).
Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631).
Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631).
CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631).
Biological Process
B cell costimulation Source: UniProtKB
Cobalamin metabolic process Source: Reactome
Cobalamin transport Source: UniProtKB
Positive regulation of B cell proliferation Source: UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Methylmalonic aciduria, transient, due to transcobalamin receptor defect (MMATC): A metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin.
Topology
Extracellular: 36-229
Helical: 230-250
Cytoplasmic: 251-282

Chen, Z., Liang, Y., Feng, X., Liang, Y., Shen, G., Huang, H., ... & Hu, Y. (2021). Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer. Materials Science and Engineering: C, 120, 111722.

Elzi, D. J., Bauta, W., Lai, S. C., Das, T., Mogare, S., & Rebel, V. I. (2021). Simultaneous knockdown of CD320 and LRP2 receptors is selectively toxic to cancer cells but not normal cells.

Boachie, J., Adaikalakoteswari, A., Goljan, I., Samavat, J., Cagampang, F. R., & Saravanan, P. (2021). Intracellular and Tissue Levels of Vitamin B12 in Hepatocytes Are Modulated by CD320 Receptor and TCN2 Transporter. International Journal of Molecular Sciences, 22(6), 3089.

Zhang, C., Chen, Y., Sun, S., Zhang, Y., Wang, L., Luo, Z., ... & Wu, Q. (2020). A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells. Elife, 9, e56059.

Guo, W., Deng, L., Chen, Z., Chen, Z., Yu, J., Liu, H., ... & Hu, Y. (2019). Vitamin B12-conjugated sericin micelles for targeting CD320-overexpressed gastric cancer and reversing drug resistance. Nanomedicine, 14(3), 353-370.

Bernard, D. J., Pangilinan, F. J., Cheng, J., Molloy, A. M., & Brody, L. C. (2018). Mice lacking the transcobalamin-vitamin B12 receptor, CD320, suffer from anemia and reproductive deficits when fed vitamin B12-deficient diet. Human molecular genetics, 27(20), 3627-3640.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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