Human CD320 ELISA Kit (V2LY-0626-LY5734)

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Tested Data
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity
8.2 mg/mL
Detection Range
20-4500 mg/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
29.0 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
CD320 molecule
Function
Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389).
Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213).
Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470).
Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631).
Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631).
CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631).
Biological Process
B cell costimulation Source: UniProtKB
Cobalamin metabolic process Source: Reactome
Cobalamin transport Source: UniProtKB
Positive regulation of B cell proliferation Source: UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Methylmalonic aciduria, transient, due to transcobalamin receptor defect (MMATC): A metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin.
Topology
Extracellular: 36-229
Helical: 230-250
Cytoplasmic: 251-282

Chen, Z., Liang, Y., Feng, X., Liang, Y., Shen, G., Huang, H., ... & Hu, Y. (2021). Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer. Materials Science and Engineering: C, 120, 111722.

Elzi, D. J., Bauta, W., Lai, S. C., Das, T., Mogare, S., & Rebel, V. I. (2021). Simultaneous knockdown of CD320 and LRP2 receptors is selectively toxic to cancer cells but not normal cells.

Boachie, J., Adaikalakoteswari, A., Goljan, I., Samavat, J., Cagampang, F. R., & Saravanan, P. (2021). Intracellular and Tissue Levels of Vitamin B12 in Hepatocytes Are Modulated by CD320 Receptor and TCN2 Transporter. International Journal of Molecular Sciences, 22(6), 3089.

Zhang, C., Chen, Y., Sun, S., Zhang, Y., Wang, L., Luo, Z., ... & Wu, Q. (2020). A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells. Elife, 9, e56059.

Guo, W., Deng, L., Chen, Z., Chen, Z., Yu, J., Liu, H., ... & Hu, Y. (2019). Vitamin B12-conjugated sericin micelles for targeting CD320-overexpressed gastric cancer and reversing drug resistance. Nanomedicine, 14(3), 353-370.

Bernard, D. J., Pangilinan, F. J., Cheng, J., Molloy, A. M., & Brody, L. C. (2018). Mice lacking the transcobalamin-vitamin B12 receptor, CD320, suffer from anemia and reproductive deficits when fed vitamin B12-deficient diet. Human molecular genetics, 27(20), 3627-3640.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

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