Mouse Recombinant CD320 protein, hFc Tag (V2LY-0526-LY7997)

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Basic Information

Expressed Host
HEK293 Cells
Protein Species
Mouse
Tag
hFc Tag
Protein Construction
This product is Mouse Recombinant CD320 protein, hFc Tag consist of Amino Acid: 1-208 and predicts a molecular mass of 46.5 kDa.
Molecule Mass
46.5 kDa
Sequence
Amino Acid: 1-208
Species
Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity
>95% as determined by SDS-PAGE
Endotoxin
Please contact us for more information.
Format
Lyophilized
Reconstitution
Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.
Buffer
Lyophilized from sterile PBS
Preservative
None
Storage
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
More Infomation

Target

Full Name
CD320 molecule
Function
Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389).
Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213).
Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470).
Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631).
Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631).
CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631).
Biological Process
B cell costimulation Source: UniProtKB
Cobalamin metabolic process Source: Reactome
Cobalamin transport Source: UniProtKB
Positive regulation of B cell proliferation Source: UniProtKB
Cellular Location
Cell membrane
Involvement in disease
Methylmalonic aciduria, transient, due to transcobalamin receptor defect (MMATC): A metabolic disorder characterized by increased blood C3-acylcarnitine levels, elevated methylmalonate and homocysteine, and low uptake of transcobalamin-bound cobalamin, but normal conversion to adenosylcobalamin and methylcobalamin.
Topology
Extracellular: 36-229
Helical: 230-250
Cytoplasmic: 251-282

Chen, Z., Liang, Y., Feng, X., Liang, Y., Shen, G., Huang, H., ... & Hu, Y. (2021). Vitamin-B12-conjugated PLGA-PEG nanoparticles incorporating miR-532-3p induce mitochondrial damage by targeting apoptosis repressor with caspase recruitment domain (ARC) on CD320-overexpressed gastric cancer. Materials Science and Engineering: C, 120, 111722.

Elzi, D. J., Bauta, W., Lai, S. C., Das, T., Mogare, S., & Rebel, V. I. (2021). Simultaneous knockdown of CD320 and LRP2 receptors is selectively toxic to cancer cells but not normal cells.

Boachie, J., Adaikalakoteswari, A., Goljan, I., Samavat, J., Cagampang, F. R., & Saravanan, P. (2021). Intracellular and Tissue Levels of Vitamin B12 in Hepatocytes Are Modulated by CD320 Receptor and TCN2 Transporter. International Journal of Molecular Sciences, 22(6), 3089.

Zhang, C., Chen, Y., Sun, S., Zhang, Y., Wang, L., Luo, Z., ... & Wu, Q. (2020). A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells. Elife, 9, e56059.

Guo, W., Deng, L., Chen, Z., Chen, Z., Yu, J., Liu, H., ... & Hu, Y. (2019). Vitamin B12-conjugated sericin micelles for targeting CD320-overexpressed gastric cancer and reversing drug resistance. Nanomedicine, 14(3), 353-370.

Bernard, D. J., Pangilinan, F. J., Cheng, J., Molloy, A. M., & Brody, L. C. (2018). Mice lacking the transcobalamin-vitamin B12 receptor, CD320, suffer from anemia and reproductive deficits when fed vitamin B12-deficient diet. Human molecular genetics, 27(20), 3627-3640.

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For research use only. Not intended for any clinical use.

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