Mouse Anti-CD86 Recombinant Antibody (CBT3775) (V2LY-0625-LY2526)

Name: Mouse Anti-CD86 Recombinant Antibody (CBT3775)
SKU: V2LY-0625-LY2526
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CD86 Recombinant Antibody (CBT3775) (V2LY-0625-LY2526) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3775

Application

Immunogen

Purified recombinant fragment of human CD86 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD86 Molecule

Entrez Gene ID

942

UniProt ID

P42081

Alternative Names

CD86 Molecule; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); CTLA-4 Counter-Receptor B7.2; CD28LG2; FUN-1; BU63; B70; B-Lymphocyte Activation Antigen B7-2;

Function

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed:7527824).
Also involved in the regulation of B cells function, plays a role in regulating the level of IgG1 produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity).
Isoform 2: Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
(Microbial infection) Acts as a receptor for adenovirus subgroup B.

Biological Process

Activation of phospholipase C activity Source: UniProtKB
Activation of protein kinase C activity Source: UniProtKB
Adaptive immune response Source: UniProtKB-KW
B cell activation Source: UniProtKB
CD40 signaling pathway Source: UniProtKB
Cell surface receptor signaling pathway Source: GO_Central
Cellular response to lipopolysaccharide Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Immune response Source: GO_Central
Negative regulation of T cell proliferation Source: GO_Central
Positive regulation of cell population proliferation Source: ProtInc
Positive regulation of immunoglobulin production Source: UniProtKB
Positive regulation of interleukin-2 production Source: UniProtKB
Positive regulation of interleukin-4 production Source: UniProtKB
Positive regulation of lymphotoxin A production Source: UniProtKB
Positive regulation of NIK/NF-kappaB signaling Source: UniProtKB
Positive regulation of protein kinase B signaling Source: Reactome
Positive regulation of T cell proliferation Source: GO_Central
Positive regulation of T-helper 2 cell differentiation Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
T cell costimulation Source: GO_Central

Cellular Location

Cell membrane

Topology

Extracellular: 24-247
Helical: 248-268
Cytoplasmic: 269-329

PTM

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Watanabe, A., Inoue, N., Watanabe, M., Yamamoto, M., Ozaki, H., Hidaka, Y., & Iwatani, Y. (2020). Increases of CD80 and CD86 expression on peripheral blood cells and their gene polymorphisms in autoimmune thyroid disease. Immunological investigations, 49(1-2), 191-203.

Tanaka, S., Ise, W., Inoue, T., Ito, A., Ono, C., Shima, Y., ... & Kurosaki, T. (2020). Tet2 and Tet3 in B cells are required to repress CD86 and prevent autoimmunity. Nature immunology, 21(8), 950-961.

de Vos, L., Grünwald, I., Bawden, E. G., Dietrich, J., Scheckenbach, K., Wiek, C., ... & Dietrich, D. (2020). The landscape of CD28, CD80, CD86, CTLA4, and ICOS DNA methylation in head and neck squamous cell carcinomas. Epigenetics, 15(11), 1195-1212.

Haabeth, O. A. W., Blake, T. R., McKinlay, C. J., Tveita, A. A., Sallets, A., Waymouth, R. M., ... & Levy, R. (2019). Local delivery of Ox40l, Cd80, and Cd86 mRNA kindles global anticancer immunity. Cancer research, 79(7), 1624-1634.

Nombela, I., Requena-Platek, R., Morales-Lange, B., Chico, V., Puente-Marin, S., Ciordia, S., ... & Ortega-Villaizan, M. D. M. (2019). Rainbow trout red blood cells exposed to viral hemorrhagic septicemia virus up-regulate antigen-processing mechanisms and MHC I&II, CD86, and CD83 antigen-presenting cell markers. Cells, 8(5), 386.

Kleinpeter, P., Remy-Ziller, C., Winter, E., Gantzer, M., Nourtier, V., Kempf, J., ... & Marchand, J. B. (2019). By binding CD80 and CD86, the vaccinia virus M2 protein blocks their interactions with both CD28 and CTLA4 and potentiates CD80 binding to PD-L1. Journal of virology, 93(11), e00207-19.

Xiong, A., Wang, J., Mao, X. L., Jiang, Y., & Fan, Y. (2019). MiR‐199a‐3p modulates the function of dendritic cells involved in transplantation tolerance by targeting CD86. Hla, 94(6), 493-503.

Pinto, B. F., Medeiros, N. I., Teixeira-Carvalho, A., Eloi-Santos, S. M., Fontes-Cal, T., Rocha, D. A., ... & Gomes, J. A. (2018). CD86 expression by monocytes influences an immunomodulatory profile in asymptomatic patients with chronic Chagas disease. Frontiers in immunology, 9, 454.

Han, L., Shen, L., Zhu, Y., & Qiu, Y. (2017). A monoclonal antibody against CD86 and its protection in a murine lupus nephritis model of chronic graft-versus-host disease. Immunopharmacology and immunotoxicology, 39(5), 285-291.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

CD86 Matched Antibody Pair (237) (CAT#: APMAB-237LY)

CD86 Matched Antibody Pair (238) (CAT#: APMAB-238LY)

CD86 Matched Antibody Pair (239) (CAT#: APMAB-239LY)

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CD86 Matched Antibody Pair (239) (CAT#: APMAB-239LY)

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Isotype control

For research use only. Not intended for any clinical use.

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