Mouse Anti-CHAF1B Recombinant Antibody (CBFYC-1799) (CBMAB-C1861-FY)

Name: Mouse Anti-CHAF1B Recombinant Antibody (CBFYC-1799)
SKU: CBMAB-C1861-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1799

Application

WB, IP, ELISA

Immunogen

Full length CAF-1 p60 of human.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

chromatin assembly factor 1, subunit B (p60)

Introduction

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

Entrez Gene ID

8208

UniProt ID

Q13112

Alternative Names

Chromatin Assembly Factor 1 Subunit B; M-Phase Phosphoprotein 7; Human Chromatin Assembly Factor-I P60 Subunit; Chromatin Assembly Factor I P60 Subunit; CAF-I 60 KDa Subunit; CAF-1 Subunit B; CAF-I P60; MPHOSPH7; CAF1P60

Function

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Biological Process

Cell cycle Source: UniProtKB-KW
Chromatin assembly Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
Nucleosome assembly Source: GO_Central

Cellular Location

Nucleus; Cytoplasm. DNA replication foci. Cytoplasmic in M phase.

PTM

Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

Zhang, Y., Yang, Y., Qiao, P., Wang, X., Yu, R., Sun, H., ... & Su, J. (2022). CHAF1b, chromatin assembly factor-1 subunit b, is essential for mouse preimplantation embryos. International Journal of Biological Macromolecules, 195, 547-557.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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