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Mouse Anti-CHAF1B Recombinant Antibody (CBYY-C1952) (CBMAB-C3390-YY)

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Published Data
CBMAB-C3390-YY-1
Mouse Anti-CHAF1B Recombinant Antibody (CBYY-C1952) (CBMAB-C3390-YY) in WB
Western blot analysis of two independent subclones expressing either shChaf1b or shCTRL treated with IPTG. Two independent experiments were performed with all four subclones with similar results, but one representative experiment is shown. ...View More
Franklin, R., Guo, Y., He, S., Chen, M., Ji, F., Zhou, X., ... & Cheloufi, S. (2022). Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity. Nature communications, 13(1), 2350.
Distributed under Open Access license CC BY 4.0, without modification.
CBMAB-C3390-YY-2
Mouse Anti-CHAF1B Recombinant Antibody (CBYY-C1952) (CBMAB-C3390-YY) in WB
Western blot time-course analysis of CHAF1A and CHAF1B in CAF-1 OFF and HOXA9 OFF conditions showing a closely matching downregulation trend of both CAF-1 subunits. Two independent experiments were performed with similar results, but one representative experiment is shown. ...View More
Franklin, R., Guo, Y., He, S., Chen, M., Ji, F., Zhou, X., ... & Cheloufi, S. (2022). Regulation of chromatin accessibility by the histone chaperone CAF-1 sustains lineage fidelity. Nature communications, 13(1), 2350.
Distributed under Open Access license CC BY 4.0, without modification.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBYY-C1952
Application
ELISA, IF, IP, WB
Immunogen
Amino acids 1-80 mapping at the N-terminus of CAF-1 p60 of human.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
chromatin assembly factor 1, subunit B (p60)
Entrez Gene ID
8208
UniProt ID
Q13112
Alternative Names
HP10433, TIG2
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Biological Process
Cell cycle Source: UniProtKB-KW
Chromatin assembly Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
Nucleosome assembly Source: GO_Central
Cellular Location
Nucleus; Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
PTM
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

Zhang, Y., Yang, Y., Qiao, P., Wang, X., Yu, R., Sun, H., ... & Su, J. (2022). CHAF1b, chromatin assembly factor-1 subunit b, is essential for mouse preimplantation embryos. International Journal of Biological Macromolecules, 195, 547-557.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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