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Mouse Anti-CHAF1B Recombinant Antibody (2G7) (CBMAB-C5209-LY)

This product is antibody recognizes CHAF1B. The antibody 2G7 immunoassay techniques such as: ELISA, WB.
See all CHAF1B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2G7
Antibody Isotype
IgG2b, κ
Application
ELISA, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
chromatin assembly factor 1, subunit B (p60)
Introduction
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008]
Entrez Gene ID
8208
UniProt ID
Q13112
Alternative Names
Chromatin Assembly Factor 1 Subunit B; M-Phase Phosphoprotein 7; Human Chromatin Assembly Factor-I P60 Subunit; Chromatin Assembly Factor I P60 Subunit; CAF-I 60 KDa Subunit; CAF-1 Subunit B; CAF-I P60; MPHOSPH7; CAF1P60;
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Biological Process
Cell cycle Source: UniProtKB-KW
Chromatin assembly Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
Nucleosome assembly Source: GO_Central
Cellular Location
Nucleus; Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
PTM
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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