Human CHAF1B ELISA Kit (V2LY-0626-LY4410)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.01 ng/mL
Detection Range
0.02-3.8 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
61.5 kDa
Components
  • Pre-coated ELISA Plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
chromatin assembly factor 1, subunit B (p60)
Function
Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Biological Process
Cell cycle Source: UniProtKB-KW
Chromatin assembly Source: UniProtKB
DNA repair Source: UniProtKB-KW
DNA replication Source: UniProtKB-KW
DNA replication-dependent nucleosome assembly Source: UniProtKB
Nucleosome assembly Source: GO_Central
Cellular Location
Nucleus; Cytoplasm. DNA replication foci. Cytoplasmic in M phase.
PTM
Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.

Zhang, Y., Yang, Y., Qiao, P., Wang, X., Yu, R., Sun, H., ... & Su, J. (2022). CHAF1b, chromatin assembly factor-1 subunit b, is essential for mouse preimplantation embryos. International Journal of Biological Macromolecules, 195, 547-557.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

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Mouse Anti-CHAF1B Recombinant Antibody (2G7) (CAT#: CBMAB-C5209-LY)

Rabbit Anti-CHAF1B Recombinant Antibody (CBWJC-2621) (CAT#: CBMAB-C3706WJ)

Mouse Anti-CHAF1B Recombinant Antibody (CBFYC-1799) (CAT#: CBMAB-C1861-FY)

Isotype control

For research use only. Not intended for any clinical use.

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