Mouse Anti-CHRM5 Recombinant Antibody (CBT3351) (V2LY-0625-LY1092)

Name: Mouse Anti-CHRM5 Recombinant Antibody (CBT3351)
SKU: V2LY-0625-LY1092
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-CHRM5 Recombinant Antibody (CBT3351) (V2LY-0625-LY1092) in ELISA

Mouse Anti-CHRM5 Recombinant Antibody (CBT3351) (V2LY-0625-LY1092) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT3351

Application

Immunogen

Purified recombinant fragment of human CHRM5 expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Cholinergic Receptor Muscarinic 5

Entrez Gene ID

1133

UniProt ID

P08912

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Biological Process

Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Chemical synaptic transmission Source: GO_Central
Dopamine transport Source: Ensembl
Gastric acid secretion Source: InterPro
G protein-coupled acetylcholine receptor signaling pathway Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Regulation of phosphatidylinositol dephosphorylation Source: Ensembl
Transmission of nerve impulse Source: Ensembl

Cellular Location

Cell membrane; Postsynaptic cell membrane

Topology

Extracellular: 1-29
Helical: 30-53
Cytoplasmic: 54-66
Helical: 67-87
Extracellular: 88-104
Helical: 105-126
Cytoplasmic: 127-146
Helical: 147-169
Extracellular: 170-191
Helical: 192-214
Cytoplasmic: 215-443
Helical: 444-464
Extracellular: 465-478
Helical: 479-498
Cytoplasmic: 499-532

Walker, L. C., Huckstep, K. L., Chen, N. A., Hand, L. J., Lindsley, C. W., Langmead, C. J., & Lawrence, A. J. (2021). Muscarinic M4 and M5 receptors in the ventral subiculum differentially modulate alcohol seeking versus consumption in male alcohol‐preferring rats. British Journal of Pharmacology.

Sakata, K., & Overacre, A. E. (2017). Promoter IV‐BDNF deficiency disturbs cholinergic gene expression of CHRNA 5, CHRM 2, and CHRM 5: effects of drug and environmental treatments. Journal of neurochemistry, 143(1), 49-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-CHRM5 Recombinant Antibody (CBT4791) (CAT#: V2LY-0625-LY1094)

Mouse Anti-CHRM5 Recombinant Antibody (EG684) (CAT#: CBMAB-EN825-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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