Human CHRM5 ELISA Kit (V2LY-0626-LY4911)

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Tested Data
Datasheet
SDS
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Basic Information

Sensitivity
0.0099 ng/mL
Detection Range
0.02-3.5 ng/mL
Sample Type
Serum, Plasma, cell culture supernates
Specificity
Human
Assay Type
Sandwich
Reactivity
Human
Assay Time
1.5 h
Molecule Mass
60.1 kDa
Components
  • Pre-coated ELISA plate: 12 wells * 8 detachable strips
  • Standard solution: 0.5ml x1
  • Standard diluent: 3ml x1
  • Streptavidin-HRP: 6ml x1
  • Stop solution: 6ml x1
  • Substrate solution A: 6ml x1
  • Substrate solution B: 6ml x1
  • Wash buffer concentrate (25x): 20ml x1
  • Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 2-8°C
More Infomation

Target

Full Name
Cholinergic Receptor Muscarinic 5
Function
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
Biological Process
Adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway Source: GO_Central
Chemical synaptic transmission Source: GO_Central
Dopamine transport Source: Ensembl
Gastric acid secretion Source: InterPro
G protein-coupled acetylcholine receptor signaling pathway Source: ProtInc
G protein-coupled receptor signaling pathway Source: Reactome
G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Source: GO_Central
Regulation of phosphatidylinositol dephosphorylation Source: Ensembl
Transmission of nerve impulse Source: Ensembl
Cellular Location
Cell membrane; Postsynaptic cell membrane
Topology
Extracellular: 1-29
Helical: 30-53
Cytoplasmic: 54-66
Helical: 67-87
Extracellular: 88-104
Helical: 105-126
Cytoplasmic: 127-146
Helical: 147-169
Extracellular: 170-191
Helical: 192-214
Cytoplasmic: 215-443
Helical: 444-464
Extracellular: 465-478
Helical: 479-498
Cytoplasmic: 499-532

Walker, L. C., Huckstep, K. L., Chen, N. A., Hand, L. J., Lindsley, C. W., Langmead, C. J., & Lawrence, A. J. (2021). Muscarinic M4 and M5 receptors in the ventral subiculum differentially modulate alcohol seeking versus consumption in male alcohol‐preferring rats. British Journal of Pharmacology.

Sakata, K., & Overacre, A. E. (2017). Promoter IV‐BDNF deficiency disturbs cholinergic gene expression of CHRNA 5, CHRM 2, and CHRM 5: effects of drug and environmental treatments. Journal of neurochemistry, 143(1), 49-64.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Chrm5 ELISA Kit (CAT#: V2LY-0626-LY2705)

Related Products

Mouse Chrm5 ELISA Kit (CAT#: V2LY-0626-LY2705)

Mouse Anti-CHRM5 Recombinant Antibody (EG684) (CAT#: CBMAB-EN825-LY)

Mouse Anti-CHRM5 Recombinant Antibody (CBT4791) (CAT#: V2LY-0625-LY1094)

Mouse Anti-CHRM5 Recombinant Antibody (CBT3351) (CAT#: V2LY-0625-LY1092)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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