Mouse Anti-CIRBP Recombinant Antibody (CBFYC-1876) (CBMAB-C1941-FY)

Name: Mouse Anti-CIRBP Recombinant Antibody (CBFYC-1876)
SKU: CBMAB-C1941-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-1876

Application

WB, ELISA, IHC-P

Immunogen

Purified recombinant fragment of human CIRBP (AA: 1-90) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:10,000
WB	1:500-1:2,000
IHC-P	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-90

More Infomation

Target

Full Name

Cold Inducible RNA Binding Protein

Introduction

CIRBP (Cold Inducible RNA Binding Protein) is a Protein Coding gene. Diseases associated with CIRBP include Cryptorchidism, Unilateral Or Bilateral. Among its related pathways are DNA Damage and Translational Control. Gene Ontology (GO) annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is RBMX.

Entrez Gene ID

1153

UniProt ID

Q14011

Alternative Names

Cold Inducible RNA Binding Protein; Cold-Inducible RNA-Binding Protein; Glycine-Rich RNA Binding Protein; A18 HnRNP; CIRP; Glycine-Rich RNA-Binding Protein CIRP; Cold Inducible RNA-Binding Protein; Testicular Tissue Protein Li 39; A18HNRNP

Function

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity).

Promotes assembly of stress granules (SGs), when overexpressed.

Biological Process

mRNA stabilization Source: UniProtKB
Positive regulation of mRNA splicing, via spliceosome Source: GO_Central
Positive regulation of translation Source: UniProtKB
Response to cold Source: ProtInc
Response to UV Source: UniProtKB
Stress granule assembly Source: UniProtKB

Cellular Location

Cytoplasm; Nucleoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins (By similarity).

PTM

Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs (By similarity).
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.

Fujita, Y., Yago, T., Asano, T., Matsumoto, H., Matsuoka, N., Temmoku, J., ... & Migita, K. (2021). Clinical relevance for circulating cold-inducible RNA-binding protein (CIRP) in patients with adult-onset Still’s disease. PloS one, 16(8), e0255493.

Ode, Y., Aziz, M., Jin, H., Arif, A., Nicastro, J. G., & Wang, P. (2019). Cold-inducible RNA-binding protein induces neutrophil extracellular traps in the lungs during sepsis. Scientific reports, 9(1), 1-11.

Hoekstra, M. M., Emmenegger, Y., Hubbard, J., & Franken, P. (2019). Cold-inducible RNA-binding protein (CIRBP) adjusts clock-gene expression and REM-sleep recovery following sleep deprivation. Elife, 8, e43400.

Liu, P., Yao, R., Shi, H., Liu, Y., Lian, S., Yang, Y., ... & Li, S. (2019). Effects of cold-inducible RNA-binding protein (CIRP) on liver glycolysis during acute cold exposure in C57BL/6 mice. International journal of molecular sciences, 20(6), 1470.

Lujan, D. A., Ochoa, J. L., & Hartley, R. S. (2018). Cold‐inducible RNA binding protein in cancer and inflammation. Wiley Interdisciplinary Reviews: RNA, 9(2), e1462.

Roilo, M., Kullmann, M. K., & Hengst, L. (2018). Cold-inducible RNA-binding protein (CIRP) induces translation of the cell-cycle inhibitor p27Kip1. Nucleic acids research, 46(6), 3198-3210.

McGinn, J., Zhang, F., Aziz, M., Yang, W. L., Nicastro, J., Coppa, G. F., & Wang, P. (2018). The protective effect of a short peptide derived from cold-inducible RNA-binding protein in renal ischemia-reperfusion injury. Shock (Augusta, Ga.), 49(3), 269.

Liao, Y., Tong, L., Tang, L., & Wu, S. (2017). The role of cold‐inducible RNA binding protein in cell stress response. International journal of cancer, 141(11), 2164-2173.

Zhong, P., & Huang, H. (2017). Recent progress in the research of cold-inducible RNA-binding protein. Future science OA, 3(4), FSO246.

Khan, M. M., Yang, W. L., Brenner, M., Bolognese, A. C., & Wang, P. (2017). Cold-inducible RNA-binding protein (CIRP) causes sepsis-associated acute lung injury via induction of endoplasmic reticulum stress. Scientific reports, 7(1), 1-13.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CIRBP Matched Antibody Pair (271) (CAT#: APMAB-271LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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