Human CIRBP ELISA Kit (2) (V2LY-0626-LY5740)

Name: Human CIRBP ELISA Kit (2)
SKU: V2LY-0626-LY5740
Rating: 5 (1 reviews)

See all CIRBP antibodies

Compare Online Inquiry

Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.008 ng/mL

Detection Range

0.015-3 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

18.6 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Cold Inducible RNA Binding Protein

Function

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity).

Promotes assembly of stress granules (SGs), when overexpressed.

Biological Process

mRNA stabilization Source: UniProtKB
Positive regulation of mRNA splicing, via spliceosome Source: GO_Central
Positive regulation of translation Source: UniProtKB
Response to cold Source: ProtInc
Response to UV Source: UniProtKB
Stress granule assembly Source: UniProtKB

Cellular Location

Cytoplasm; Nucleoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins (By similarity).

PTM

Methylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs (By similarity).
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation.

Fujita, Y., Yago, T., Asano, T., Matsumoto, H., Matsuoka, N., Temmoku, J., ... & Migita, K. (2021). Clinical relevance for circulating cold-inducible RNA-binding protein (CIRP) in patients with adult-onset Still’s disease. PloS one, 16(8), e0255493.

Ode, Y., Aziz, M., Jin, H., Arif, A., Nicastro, J. G., & Wang, P. (2019). Cold-inducible RNA-binding protein induces neutrophil extracellular traps in the lungs during sepsis. Scientific reports, 9(1), 1-11.

Hoekstra, M. M., Emmenegger, Y., Hubbard, J., & Franken, P. (2019). Cold-inducible RNA-binding protein (CIRBP) adjusts clock-gene expression and REM-sleep recovery following sleep deprivation. Elife, 8, e43400.

Liu, P., Yao, R., Shi, H., Liu, Y., Lian, S., Yang, Y., ... & Li, S. (2019). Effects of cold-inducible RNA-binding protein (CIRP) on liver glycolysis during acute cold exposure in C57BL/6 mice. International journal of molecular sciences, 20(6), 1470.

Lujan, D. A., Ochoa, J. L., & Hartley, R. S. (2018). Cold‐inducible RNA binding protein in cancer and inflammation. Wiley Interdisciplinary Reviews: RNA, 9(2), e1462.

Roilo, M., Kullmann, M. K., & Hengst, L. (2018). Cold-inducible RNA-binding protein (CIRP) induces translation of the cell-cycle inhibitor p27Kip1. Nucleic acids research, 46(6), 3198-3210.

McGinn, J., Zhang, F., Aziz, M., Yang, W. L., Nicastro, J., Coppa, G. F., & Wang, P. (2018). The protective effect of a short peptide derived from cold-inducible RNA-binding protein in renal ischemia-reperfusion injury. Shock (Augusta, Ga.), 49(3), 269.

Liao, Y., Tong, L., Tang, L., & Wu, S. (2017). The role of cold‐inducible RNA binding protein in cell stress response. International journal of cancer, 141(11), 2164-2173.

Zhong, P., & Huang, H. (2017). Recent progress in the research of cold-inducible RNA-binding protein. Future science OA, 3(4), FSO246.

Khan, M. M., Yang, W. L., Brenner, M., Bolognese, A. C., & Wang, P. (2017). Cold-inducible RNA-binding protein (CIRP) causes sepsis-associated acute lung injury via induction of endoplasmic reticulum stress. Scientific reports, 7(1), 1-13.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human CIRBP ELISA Kit (2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Cirbp ELISA Kit (CAT#: V2LY-0626-LY1570)

Mouse Cirbp ELISA Kit (CAT#: V2LY-0626-LY2198)

Human CIRBP ELISA Kit (CAT#: V2LY-0626-LY4420)

CIRBP Matched Antibody Pair (271) (CAT#: APMAB-271LY)

Antibody Pairs

CIRBP Matched Antibody Pair (271) (CAT#: APMAB-271LY)

Related Products

Rabbit Anti-CIRBP Recombinant Antibody (CP0315) (CAT#: CBMAB-CP0315-LY)

Mouse Anti-CIRBP Recombinant Antibody (CBFYC-1876) (CAT#: CBMAB-C1941-FY)

Mouse Anti-CIRBP Recombinant Antibody (1C9) (CAT#: CBMAB-C3784-LY)

CIRBP Matched Antibody Pair (271) (CAT#: APMAB-271LY)

Mouse Cirbp ELISA Kit (CAT#: V2LY-0626-LY2198)

Mouse Anti-CIRBP Recombinant Antibody (CBT4787) (CAT#: V2LY-0625-LY682)

Rat Cirbp ELISA Kit (CAT#: V2LY-0626-LY1570)

Human CIRBP ELISA Kit (CAT#: V2LY-0626-LY4420)

Mouse Anti-CIRBP Recombinant Antibody (CBT2437) (CAT#: V2LY-0625-LY658)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry