Mouse Anti-CLEC14A Recombinant Antibody (C8488) (V2LY-1206-LY88)

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Basic Information

Host Animal
Mouse
Clone
C8488
Application
FC, IC
Immunogen
Recombinant human CLEC14A Glu22-Ala397.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG2b
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
FC2.5 µg/10^6 cells
IF(ICC)8-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS, Trehalose
Preservative
None
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
C-Type Lectin Domain Containing 14A
Entrez Gene ID
UniProt ID
Biological Process
Cell migration Source: GO_Central
Cell migration involved in sprouting angiogenesis Source: Ensembl
Lymphangiogenesis Source: Ensembl
Vascular endothelial growth factor receptor-2 signaling pathway Source: GO_Central
Vascular endothelial growth factor receptor-3 signaling pathway Source: GO_Central
Cellular Location
Membrane
Topology
Extracellular: 22-397
Helical: 398-418
Cytoplasmic: 419-490

Qiao, C., Qiu, Y., Zou, Y., Hua, C., Li, Y., Jin, Y., & Lin, X. (2021). Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations. Chinese Journal of Plastic and Reconstructive Surgery.

Sandoval, D. R., Toledo, A. G., Painter, C. D., Tota, E. M., Sheikh, M. O., West, A. M., ... & Esko, J. D. (2020). Proteomics-based screening of the endothelial heparan sulfate interactome reveals that C-type lectin 14a (CLEC14A) is a heparin-binding protein. Journal of Biological Chemistry, 295(9), 2804-2821.

Kim, Y., Lee, S., Zhang, H., Lee, S., Kim, H., Kim, Y., ... & Kwon, Y. G. (2020). CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation. Journal of neuroinflammation, 17(1), 1-14.

Bocci, M., Sjölund, J., Kurzejamska, E., Lindgren, D., Marzouka, N. A. D., Bartoschek, M., ... & Pietras, K. (2019). Activin receptor-like kinase 1 is associated with immune cell infiltration and regulates CLEC14A transcription in cancer. Angiogenesis, 22(1), 117-131.

Kim, T. K., Park, C. S., Jang, J., Kim, M. R., Na, H. J., Lee, K., ... & Lee, S. (2018). Inhibition of VEGF‐dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC 14a. Molecular oncology, 12(3), 356-372.

Priya, S. K., Kumar, K., Hiran, K. R., Bindhu, M. R., Nagare, R. P., Vijaykumar, D. K., & Ganesan, T. S. (2017). Expression of a novel endothelial marker, C-type lectin 14A, in epithelial ovarian cancer and its prognostic significance. International journal of clinical oncology, 22(1), 107-117.

Jang, J., Kim, M. R., Kim, T. K., Lee, W. R., Kim, J. H., Heo, K., & Lee, S. (2017). CLEC14a-HSP70-1A interaction regulates HSP70-1A-induced angiogenesis. Scientific reports, 7(1), 1-12.

Lee, S., Rho, S. S., Park, H., Park, J. A., Kim, J., Lee, I. K., ... & Kwon, Y. G. (2017). Carbohydrate-binding protein CLEC14A regulates VEGFR-2–and VEGFR-3–dependent signals during angiogenesis and lymphangiogenesis. The Journal of clinical investigation, 127(2), 457-471.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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