Mouse Recombinant CLEC14A protein, His Tag (V2LY-0526-LY8125)

Name: Mouse Recombinant CLEC14A protein, His Tag
SKU: V2LY-0526-LY8125
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Mouse

Tag

His Tag

Protein Construction

This product is Mouse Recombinant CLEC14A protein, His Tag consist of Amino Acid: 1-386 and predicts a molecular mass of 40.6 kDa.

Molecule Mass

40.6 kDa

Sequence

Amino Acid: 1-386

Species

Mouse

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

C-Type Lectin Domain Containing 14A

Biological Process

Cell migration Source: GO_Central
Cell migration involved in sprouting angiogenesis Source: Ensembl
Lymphangiogenesis Source: Ensembl
Vascular endothelial growth factor receptor-2 signaling pathway Source: GO_Central
Vascular endothelial growth factor receptor-3 signaling pathway Source: GO_Central

Cellular Location

Membrane

Topology

Extracellular: 22-397
Helical: 398-418
Cytoplasmic: 419-490

Qiao, C., Qiu, Y., Zou, Y., Hua, C., Li, Y., Jin, Y., & Lin, X. (2021). Bleomycin inhibits CLEC14A to attenuate the progression of extracranial arteriovenous malformations. Chinese Journal of Plastic and Reconstructive Surgery.

Sandoval, D. R., Toledo, A. G., Painter, C. D., Tota, E. M., Sheikh, M. O., West, A. M., ... & Esko, J. D. (2020). Proteomics-based screening of the endothelial heparan sulfate interactome reveals that C-type lectin 14a (CLEC14A) is a heparin-binding protein. Journal of Biological Chemistry, 295(9), 2804-2821.

Kim, Y., Lee, S., Zhang, H., Lee, S., Kim, H., Kim, Y., ... & Kwon, Y. G. (2020). CLEC14A deficiency exacerbates neuronal loss by increasing blood-brain barrier permeability and inflammation. Journal of neuroinflammation, 17(1), 1-14.

Bocci, M., Sjölund, J., Kurzejamska, E., Lindgren, D., Marzouka, N. A. D., Bartoschek, M., ... & Pietras, K. (2019). Activin receptor-like kinase 1 is associated with immune cell infiltration and regulates CLEC14A transcription in cancer. Angiogenesis, 22(1), 117-131.

Kim, T. K., Park, C. S., Jang, J., Kim, M. R., Na, H. J., Lee, K., ... & Lee, S. (2018). Inhibition of VEGF‐dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC 14a. Molecular oncology, 12(3), 356-372.

Priya, S. K., Kumar, K., Hiran, K. R., Bindhu, M. R., Nagare, R. P., Vijaykumar, D. K., & Ganesan, T. S. (2017). Expression of a novel endothelial marker, C-type lectin 14A, in epithelial ovarian cancer and its prognostic significance. International journal of clinical oncology, 22(1), 107-117.

Jang, J., Kim, M. R., Kim, T. K., Lee, W. R., Kim, J. H., Heo, K., & Lee, S. (2017). CLEC14a-HSP70-1A interaction regulates HSP70-1A-induced angiogenesis. Scientific reports, 7(1), 1-12.

Lee, S., Rho, S. S., Park, H., Park, J. A., Kim, J., Lee, I. K., ... & Kwon, Y. G. (2017). Carbohydrate-binding protein CLEC14A regulates VEGFR-2–and VEGFR-3–dependent signals during angiogenesis and lymphangiogenesis. The Journal of clinical investigation, 127(2), 457-471.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rat Recombinant CLEC14A protein, hFc Tag (CAT#: V2LY-0526-LY9287)

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Mouse Anti-CLEC14A Recombinant Antibody (C8488) (CAT#: V2LY-1206-LY88)

Rabbit Anti-CLEC14A Recombinant Antibody (CBFYC-1912) (CAT#: V2LY-1206-LY90)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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