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Mouse Anti-CLTB Recombinant Antibody (CBCNC-029) (V2LY-1206-LY310)


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Published Data
V2LY-1206-LY310-1
Mouse Anti-CLTB Recombinant Antibody (CBCNC-029) (V2LY-1206-LY310) in IF
Super-resolution imaging of 20 immunostained proteins in cultured neurons after fixation with PFA or glyoxal. ...View More
Richter, K. N., Revelo, N. H., Seitz, K. J., Helm, M. S., Sarkar, D., Saleeb, R. S., ... & Rizzoli, S. O. (2018). Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy. The EMBO journal, 37(1), 139-159.
Distributed under Open Access license CC BY 3.0, without modification.
V2LY-1206-LY310-2
Mouse Anti-CLTB Recombinant Antibody (CBCNC-029) (V2LY-1206-LY310) in WB
Western blots of Clathrin Light Chain from the whole brain samples of Aux KO and WT mice (3 and 9 months). ...View More
Vidyadhara, D. J., Somayaji, M., Wade, N., Yücel, B., Zhao, H., Shashaank, N., ... & Chandra, S. S. (2023). Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease. Cell reports, 42(3).
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY310-3
Mouse Anti-CLTB Recombinant Antibody (CBCNC-029) (V2LY-1206-LY310) in IHC
Representative images of dorsal striatum immunostained for clathrin light chain (CLC), endophilin-A1 (Endo-A1) and Hsc70, in WT and Aux KO mice at 3 and 9 months of age. Scale bar: 40 µm. ...View More
Vidyadhara, D. J., Somayaji, M., Wade, N., Yücel, B., Zhao, H., Shashaank, N., ... & Chandra, S. S. (2023). Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson's disease. Cell reports, 42(3).
Distributed under Open Access license CC BY 4.0, without modification.
V2LY-1206-LY310-4
Mouse Anti-CLTB Recombinant Antibody (CBCNC-029) (V2LY-1206-LY310) in IHC
Representative electron micrographs of a rod (A) and cone photoreceptor (B) terminal in the light adapted mouse retina. Clathrin-coated pits (CCP) budding off from the plasma membrane are demarcated with dashed lines and magnified in the insets. Arrowheads point to synaptic ribbons; arrows to CCP remote from synaptic ribbons. ...View More
Fuchs, M., Brandstätter, J. H., & Regus-Leidig, H. (2014). Evidence for a Clathrin-independent mode of endocytosis at a continuously active sensory synapse. Frontiers in cellular neuroscience, 8, 60.
Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Zebrafish
Clone
CBCNC-029
Antibody Isotype
IgG1, κ
Application
IF, IHC, WB, IP

Basic Information

Immunogen
Synthetic peptide corresponding to AA 156 to 173 from rat Clathrin light chainB.
Host Species
Mouse
Specificity
Human, Mouse, Rat, Zebrafish
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:1,000-1:10,000
IF(ICC)1:500-1:1,000
IHC-P1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Clathrin Light Chain B
Entrez Gene ID
Human1212
Mouse74325
Rat116561
UniProt ID
HumanP09497
MouseQ6IRU5
RatP08082
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Biological Process
Clathrin coat assembly Source: GO_Central
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Membrane organization Source: Reactome
Cellular Location
Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles.
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For research use only. Not intended for any clinical use.

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