Mouse Anti-CLTB Recombinant Antibody (CBLNC-201) (V2LY-1206-LY306)

Name: Mouse Anti-CLTB Recombinant Antibody (CBLNC-201)
SKU: V2LY-1206-LY306
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CLTB Recombinant Antibody (CBLNC-201) (V2LY-1206-LY306) in IF

(A-H) Medial and trans-Golgi proteins (0.25 ≤ LQ < 1.0), except Arf1, localize to the cisternal rim. En face and side view images are shown. ...View More

Tie, H. C., Ludwig, A., Sandin, S., & Lu, L. (2018). The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack. Elife, 7, e41301.

Distributed under Open Access license CC BY 4.0, without modification.

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBLNC-201

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen

Amino acids 41-100 mapping within an internal region of Clathrin HC of human.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

0.1% gelatin

Preservative

<0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Clathrin Light Chain B

Entrez Gene ID

1212

UniProt ID

P09497

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Biological Process

Clathrin coat assembly Source: GO_Central
Clathrin-dependent endocytosis Source: GO_Central
Intracellular protein transport Source: InterPro
Membrane organization Source: Reactome

Cellular Location

Cytoplasmic vesicle membrane; Coated pit. Cytoplasmic face of coated pits and vesicles.

More Infomation

References

Mukenhirn, M., Muraca, F., Bucher, D., Asberger, E., Cappio Barazzone, E., Cavalcanti-Adam, E. A., & Boulant, S. (2021). Role of Clathrin Light Chains in Regulating Invadopodia Formation. Cells, 10(2), 451.

Redlingshöfer, L., & Brodsky, F. M. (2021). Antagonistic regulation controls clathrin-mediated endocytosis: AP2 adaptor facilitation vs restraint from clathrin light chains. Cells & Development, 203714.

Redlingshöfer, L., McLeod, F., Chen, Y., Camus, M. D., Burden, J. J., Palomer, E., ... & Brodsky, F. M. (2020). Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo. Proceedings of the National Academy of Sciences, 117(38), 23527-23538.

Khasa, R., Vaidya, A., Vrati, S., & Kalia, M. (2019). Membrane trafficking RNA interference screen identifies a crucial role of the clathrin endocytic pathway and ARP2/3 complex for Japanese encephalitis virus infection in HeLa cells. Journal of General Virology, 100(2), 176-186.

Li, Y., Liu, H., Wu, K., Liu, H., Huang, T., Chen, Z. J., ... & Zhao, H. (2019). Melatonin promotes human oocyte maturation and early embryo development by enhancing clathrin‐mediated endocytosis. Journal of Pineal Research, 67(3), e12601.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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