Rabbit Anti-CNN1 Recombinant Antibody (
2H1) (V2LY-0725-LY673)

Name: Rabbit Anti-CNN1 Recombinant Antibody (2H1)
SKU: V2LY-0725-LY673
Rating: 5 (1 reviews)

See all CNN1 antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-CNN1 Recombinant Antibody (2H1) (V2LY-0725-LY673) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, Mouse lung tissue
All lanes: CNN1 antibody at 1.05μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 32 KDa
Observed band size: 34 KDa

Rabbit Anti-CNN1 Recombinant Antibody (2H1) (V2LY-0725-LY673) in IHC

IHC image of V2LY-0725-LY673 diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

2H1

Application

ELISA, WB, IHC

Immunogen

A synthesized peptide derived from human CNN1.

Host Species

Rabbit

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Calponin 1

Entrez Gene ID

1264

UniProt ID

P51911

Function

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Biological Process

Actomyosin structure organization Source: InterPro
Negative regulation of vascular associated smooth muscle cell proliferation Source: BHF-UCL
Regulation of smooth muscle contraction Source: UniProtKB

Cellular Location

Cytoskeleton; Focal adhesion

Choo, Y. Y., Sakai, T., Komatsu, S., Ikebe, R., Jeffers, A., Singh, K. P., ... & Ikebe, M. (2022). Calponin 1 contributes to myofibroblast differentiation of human pleural mesothelial cells. American Journal of Physiology-Lung Cellular and Molecular Physiology.

Mamoor, S. (2021). Differential expression of calponin 1 in cancers of the breast.

Liu, W., Fu, X., & Li, R. (2021). CNN1 regulates the DKK1/Wnt/β‑catenin/c‑myc signaling pathway by activating TIMP2 to inhibit the invasion, migration and EMT of lung squamous cell carcinoma cells. Experimental and therapeutic medicine, 22(2), 1-9.

Wang, Z., Li, T. E., Chen, M., Pan, J. J., & Shen, K. W. (2020). miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway. Aging (Albany NY), 12(2), 1867.

Feng, H. Z., Wang, H., Takahashi, K., & Jin, J. P. (2019). Double deletion of calponin 1 and calponin 2 in mice decreases systemic blood pressure with blunted length-tension response of aortic smooth muscle. Journal of molecular and cellular cardiology, 129, 49-57.

Zhao, H., Yang, K., Liu, Q., Hu, J., Wu, W., & Wang, J. (2019). Expression of calponin-1 and its pathogenic role in systemic sclerosis. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 39(3), 279-285.

Cetin, O., Karaman, E., Boza, B., Cim, N., & Sahin, H. G. (2018). Maternal serum calponin 1 level as a biomarker for the short-term prediction of preterm birth in women with threatened preterm labor. The Journal of Maternal-Fetal & Neonatal Medicine, 31(2), 216-222.

Xu, H., Wu, F., Zhang, H., Yang, C., Li, K., Wang, H., ... & Dai, Z. (2017). Actin cytoskeleton mediates BMP2-Smad signaling via calponin 1 in preosteoblast under simulated microgravity. Biochimie, 138, 184-193.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-CNN1 Recombinant Antibody (
2H1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

CNN1 Matched Antibody Pair (282) (CAT#: APMAB-282LY)

Related Products

Mouse Anti-CNN1 Recombinant Antibody (CBXC-1376) (CAT#: V2LY-1206-LY433)

Mouse Anti-CNN1 Recombinant Antibody (CBWJC-2854) (CAT#: V2LY-1206-LY438)

Rabbit Anti-CNN1 Recombinant Antibody (CBWJC-2047) (CAT#: V2LY-1206-LY436)

Mouse Anti-CNN1 Recombinant Antibody (2F5-1H4) (CAT#: CBMAB-C5108-LY)

Rabbit Anti-CNN1 Recombinant Antibody (CBCNC-224) (CAT#: V2LY-1206-LY439)

Mouse Anti-CNN1 Recombinant Antibody (CBYY-C0677) (CAT#: V2LY-1206-LY431)

Mouse Anti-CNN1 Recombinant Antibody (CBFYC-1987) (CAT#: V2LY-1206-LY430)

Mouse Anti-CNN1 Recombinant Antibody (CBT4833) (CAT#: V2LY-0625-LY71)

Mouse Anti-CNN1 Recombinant Antibody (CBXC-2706) (CAT#: V2LY-1206-LY432)

Mouse Anti-CNN1 Recombinant Antibody (CBXC-0489) (CAT#: CBMAB-C5142-CQ)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry