Mouse Anti-CXCR3 Recombinant Antibody (CBFYC-2537) (V2LY-0125-LY784)

Name: Mouse Anti-CXCR3 Recombinant Antibody (CBFYC-2537)
SKU: V2LY-0125-LY784
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYC-2537

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 1-95 of human CXCR-3.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/mL

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

C-X-C Motif Chemokine Receptor 3

Entrez Gene ID

2833

UniProt ID

P49682

Alternative Names

C-X-C Motif Chemokine Receptor 3; G Protein-Coupled Receptor 9; Interferon-Inducible Protein 10 Receptor; Chemokine (C-X-C Motif) Receptor 3; IP-10 Receptor; CKR-L2; GPR9; C-X-C Chemokine Receptor Type 3; Chemokine Receptor 3; CD183 Antigen;

Function

Isoform 1:
Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of human mesangial cells (HMC) through a heterotrimeric G-protein signaling pathway (PubMed:12782716).

Binds to CCL21. Probably promotes cell chemotaxis response.

Isoform 2:
Receptor for the C-X-C chemokine CXCL4 and also mediates the inhibitory activities of CXCL9, CXCL10 and CXCL11 on the proliferation, survival and angiogenic activity of human microvascular endothelial cells (HMVEC) through a cAMP-mediated signaling pathway (PubMed:12782716).

Does not promote cell chemotaxis respons. Interaction with CXCL4 or CXCL10 leads to activation of the p38MAPK pathway and contributes to inhibition of angiogenesis. Overexpression in renal cancer cells down-regulates expression of the anti-apoptotic protein HMOX1 and promotes apoptosis.

Isoform 3:
Mediates the activity of CXCL11.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: UniProtKB
Angiogenesis Source: UniProtKB-KW
Apoptotic process Source: UniProtKB-KW
Calcium-mediated signaling Source: GO_Central
Cell adhesion Source: ProtInc
Cell chemotaxis Source: GO_Central
Cell surface receptor signaling pathway Source: BHF-UCL
Chemokine (C-C motif) ligand 11 production Source: UniProtKB
Chemotaxis Source: ProtInc
G protein-coupled receptor signaling pathway Source: UniProtKB
Immune response Source: GO_Central
Inflammatory response Source: InterPro
Negative regulation of angiogenesis Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of execution phase of apoptosis Source: UniProtKB
Positive regulation of angiogenesis Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of chemotaxis Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: GO_Central
Positive regulation of execution phase of apoptosis Source: UniProtKB
Positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of cell adhesion Source: UniProtKB
Regulation of leukocyte migration Source: InterPro
T cell chemotaxis Source: Ensembl

Cellular Location

Isoform 1&2: Cell membrane

Topology

Extracellular: 1-53
Helical: 54-80
Cytoplasmic: 81-89
Helical: 90-110
Extracellular: 111-125
Helical: 126-147
Cytoplasmic: 148-169
Helical: 170-189
Extracellular: 190-212
Helical: 213-233
Cytoplasmic: 234-255
Helical: 256-277
Extracellular: 278-298
Helical: 299-321
Cytoplasmic: 322-368

PTM

Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.
N-glycosylated.

Kundu, N., Ma, X., Brox, R., Fan, X., Kochel, T., Reader, J., ... & Fulton, A. (2019). The chemokine receptor CXCR3 isoform B drives breast cancer stem cells. Breast Cancer: Basic and Clinical Research, 13, 1178223419873628.

Brox, R., Milanos, L., Saleh, N., Baumeister, P., Buschauer, A., Hofmann, D., ... & Tschammer, N. (2018). Molecular mechanisms of biased and probe-dependent signaling at CXC-motif chemokine receptor CXCR3 induced by negative allosteric modulators. Molecular Pharmacology, 93(4), 309-322.

Kim, B., Lee, J. H., Jin, W. J., Kim, H. H., Ha, H., & Lee, Z. H. (2018). JN-2, a CXC motif chemokine receptor 3 antagonist, ameliorates arthritis progression in an animal model. European Journal of Pharmacology, 823, 1-10.

Metzemaekers, M., Vanheule, V., Janssens, R., Struyf, S., & Proost, P. (2018). Overview of the mechanisms that may contribute to the non-redundant activities of interferon-inducible CXC chemokine receptor 3 ligands. Frontiers in immunology, 8, 1970.

Liu, Q. Z., Ma, W. T., Yang, J. B., Zhao, Z. B., Yan, K., Yao, Y., ... & Lian, Z. X. (2018). The CXC chemokine receptor 3 inhibits autoimmune cholangitis via CD8+ T cells but promotes colitis via CD4+ T cells. Frontiers in immunology, 9, 1090.

Weigold, F., Günther, J., Pfeiffenberger, M., Cabral-Marques, O., Siegert, E., Dragun, D., ... & Riemekasten, G. (2018). Antibodies against chemokine receptors CXCR3 and CXCR4 predict progressive deterioration of lung function in patients with systemic sclerosis. Arthritis research & therapy, 20(1), 1-9.

Ma, H. D., Ma, W. T., Liu, Q. Z., Zhao, Z. B., Tsuneyama, K., Gao, J. M., ... & Lian, Z. X. (2017). Chemokine receptor CXCR3 deficiency exacerbates murine autoimmune cholangitis by promoting pathogenic CD8+ T cell activation. Journal of autoimmunity, 78, 19-28.

Priyathilaka, T. T., Oh, M., Bathige, S. D. N. K., De Zoysa, M., & Lee, J. (2017). Two distinct CXC chemokine receptors (CXCR3 and CXCR4) from the big-belly seahorse Hippocampus abdominalis: Molecular perspectives and immune defensive role upon pathogenic stress. Fish & Shellfish Immunology, 65, 59-70.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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