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Mouse Anti-DGKZ Recombinant Antibody (1A6) (CBMAB-D0795-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Diacylglycerol Kinase Zeta (DGKZ). The antibody can be used for immunoassay techniques, such as WB, IHC.
See all DGKZ antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A6
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 734-808 of human DGKZ(NP_003637) produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Diacylglycerol Kinase Zeta
Introduction
DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified.
Entrez Gene ID
8525
UniProt ID
Q13574
Alternative Names
Diacylglycerol Kinase Zeta; Diacylglycerol Kinase, Zeta 104kDa; Diglyceride Kinase Zeta; DAG Kinase Zeta; EC 2.7.1.107; DGK-ZETA; DAGK6; HDGKzeta; DAGK5;
Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095).

Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:9159104, PubMed:19744926, PubMed:22108654).

Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129).

The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity).

By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668).

Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).

Isoform 1:
Regulates RASGRP1 activity.

Isoform 2:
Does not regulate RASGRP1 activity.
Biological Process
Cell migration Source: UniProtKB
Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Mitotic G1 DNA damage checkpoint Source: UniProtKB
Negative regulation of mitotic cell cycle Source: UniProtKB
Negative regulation of T cell receptor signaling pathway Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Positive regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: UniProtKB
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Regulation of synaptic transmission, glutamatergic Source: GO_Central
Cellular Location
Cytosol; Nucleus; Cell membrane; Lamellipodium
PTM
Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.

Zhao, Y., Sun, H., Li, X., Liu, Q., Liu, Y., Hou, Y., & Jin, W. (2022). DGKZ promotes TGFβ signaling pathway and metastasis in triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFβR2. Cell death & disease, 13(2), 1-12.

Liu, K., Xue, B., Bai, G., & Zhang, W. (2021). Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest. Bioengineered, 12(1), 1517-1529.

Li, H., Dong, C., Tian, Y., Li, X., Wang, B., Zhai, D., ... & Chao, X. (2019). Knockdown of diacylglycerol kinase zeta (DGKZ) induces apoptosis and G2/M phase arrest in human acute myeloid leukemia HL-60 cells through MAPK/survivin/caspase pathway. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 74(7), 418-422.

Yu, W., Tang, L., Lin, F., Yao, Y., & Shen, Z. (2019). DGKZ acts as a potential oncogene in osteosarcoma proliferation through its possible interaction with ERK1/2 and MYC pathway. Frontiers in Oncology, 8, 655.

Alinaghi, S., Alehabib, E., Johari, A. H., Vafaei, F., Salehi, S., Darvish, H., & Ghaedi, H. (2019). Expression analysis and genotyping of dgkz: a gwas-derived risk gene for schizophrenia. Molecular biology reports, 46(4), 4105-4111.

Jing, W., Gershan, J. A., Holzhauer, S., Weber, J., Palen, K., McOlash, L., ... & Riese, M. J. (2017). T cells deficient in diacylglycerol kinase ζ are resistant to PD-1 inhibition and help create persistent host immunity to leukemia. Cancer Research, 77(20), 5676-5686.

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For research use only. Not intended for any clinical use.

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