Rat Recombinant DGKZ, Active protein, His Tag (V2LY-0526-LY9330)

Name: Rat Recombinant DGKZ, Active protein, His Tag
SKU: V2LY-0526-LY9330
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

Baculovirus-Insect Cells

Protein Species

Rat

Tag

His Tag

Protein Construction

This product is Rat Recombinant DGKZ, Active protein, His Tag consist of Amino Acid: Full Length and predicts a molecular mass of 127 kDa.

Molecule Mass

127 kDa

Sequence

Amino Acid: Full Length

Species

Rat

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

Batch dependent.

Endotoxin

Please contact us for more information.

Format

Liquid

Buffer

Tris, NaCl, Glutathione, EDTA, DTT, PMSF, Glycerol

Preservative

None

Storage

Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

More Infomation

Target

Full Name

Diacylglycerol Kinase Zeta

Function

Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095).

Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:9159104, PubMed:19744926, PubMed:22108654).

Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129).

The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity).

By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668).

Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity).

Isoform 1:
Regulates RASGRP1 activity.

Isoform 2:
Does not regulate RASGRP1 activity.

Biological Process

Cell migration Source: UniProtKB
Diacylglycerol metabolic process Source: UniProtKB
Glycerolipid metabolic process Source: BHF-UCL
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Mitotic G1 DNA damage checkpoint Source: UniProtKB
Negative regulation of mitotic cell cycle Source: UniProtKB
Negative regulation of T cell receptor signaling pathway Source: UniProtKB
Phosphatidic acid biosynthetic process Source: UniProtKB
Platelet activation Source: Reactome
Positive regulation of 1-phosphatidylinositol-4-phosphate 5-kinase activity Source: UniProtKB
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Regulation of synaptic transmission, glutamatergic Source: GO_Central

Cellular Location

Cytosol; Nucleus; Cell membrane; Lamellipodium

PTM

Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.

Zhao, Y., Sun, H., Li, X., Liu, Q., Liu, Y., Hou, Y., & Jin, W. (2022). DGKZ promotes TGFβ signaling pathway and metastasis in triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFβR2. Cell death & disease, 13(2), 1-12.

Liu, K., Xue, B., Bai, G., & Zhang, W. (2021). Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest. Bioengineered, 12(1), 1517-1529.

Li, H., Dong, C., Tian, Y., Li, X., Wang, B., Zhai, D., ... & Chao, X. (2019). Knockdown of diacylglycerol kinase zeta (DGKZ) induces apoptosis and G2/M phase arrest in human acute myeloid leukemia HL-60 cells through MAPK/survivin/caspase pathway. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 74(7), 418-422.

Yu, W., Tang, L., Lin, F., Yao, Y., & Shen, Z. (2019). DGKZ acts as a potential oncogene in osteosarcoma proliferation through its possible interaction with ERK1/2 and MYC pathway. Frontiers in Oncology, 8, 655.

Alinaghi, S., Alehabib, E., Johari, A. H., Vafaei, F., Salehi, S., Darvish, H., & Ghaedi, H. (2019). Expression analysis and genotyping of dgkz: a gwas-derived risk gene for schizophrenia. Molecular biology reports, 46(4), 4105-4111.

Jing, W., Gershan, J. A., Holzhauer, S., Weber, J., Palen, K., McOlash, L., ... & Riese, M. J. (2017). T cells deficient in diacylglycerol kinase ζ are resistant to PD-1 inhibition and help create persistent host immunity to leukemia. Cancer Research, 77(20), 5676-5686.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant DGKZ, Active protein, GST Tag (CAT#: V2LY-0526-LY3576)

Human Recombinant DGKZ, Active protein, His Tag (CAT#: V2LY-0526-LY3577)

Mouse Recombinant DGKZ, Active protein, His Tag (CAT#: V2LY-0526-LY8197)

DGKZ Matched Antibody Pair (339) (CAT#: APMAB-339LY)

Antibody Pairs

DGKZ Matched Antibody Pair (339) (CAT#: APMAB-339LY)

Related Products

DGKZ Matched Antibody Pair (339) (CAT#: APMAB-339LY)

Human Recombinant DGKZ, Active protein, His Tag (CAT#: V2LY-0526-LY3577)

Mouse Recombinant DGKZ, Active protein, His Tag (CAT#: V2LY-0526-LY8197)

Mouse Anti-DGKZ Recombinant Antibody (1A6) (CAT#: V2LY-0425-LY254)

Human Recombinant DGKZ, Active protein, GST Tag (CAT#: V2LY-0526-LY3576)

Mouse Anti-DGKZ Recombinant Antibody (1A6) (CAT#: CBMAB-D0795-YC)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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