Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271)

Name: Mouse Anti-DHFR Recombinant Antibody (D0823)
SKU: V2LY-0425-LY271
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-1) in WB

MDA-MB-468 lysates were treated with 10 μM of each compound and incubated at 45 °C. Lysates were electrophoretically separated, and DHFR was detected via Western blot. DHFR bands were normalized to the thermostable SOD1 control. ...View More

Brown, J. I., Wang, P., Wong, A. Y., Petrova, B., Persaud, R., Soukhtehzari, S., ... & Page, B. D. (2023). Cycloguanil and analogues potently target DHFR in cancer cells to elicit anti-cancer activity. Metabolites, 13(2), 151.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-2) in IF

The effect of silencing of CSNK2A1 on localisation of DHFR (e) and TS (d) in A-549 cells. Detection of TS, DHFR and CK2α in control cells are shown in (a), (b), (c), respectively. The cells were treated with 100 nM CSNK2A1 siRNA for 72 h (d-f), fixed, and after blocking, probed with the primary anti-DHFR or anti-TS antibodies. ...View More

Wińska, P., Sobiepanek, A., Pawlak, K., Staniszewska, M., & Cieśla, J. (2023). Phosphorylation of Thymidylate Synthase and Dihydrofolate Reductase in Cancer Cells and the Effect of CK2α Silencing. International Journal of Molecular Sciences, 24(3), 3023.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-3) in WB

DHFR protein expression in Mero95 and RN5 WT, KD and rescue cells. Protein level is represented relative to the respective WT. ...View More

Hariharan, A., Qi, W., Rehrauer, H., Wu, L., Ronner, M., Wipplinger, M., ... & Felley-Bosco, E. (2022). Heterogeneous RNA editing and influence of ADAR2 on mesothelioma chemoresistance and the tumor microenvironment. Molecular Oncology, 16(22), 3949-3974.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-4) in WB

Western blot analysis of DHFR in CHO-K1 WT, DHFR− clone 4, 13, 14, and 16. β-actin was used as a loading control. ...View More

Min, H., Kim, S. M., Kim, D., Lee, S., Lee, S., & Lee, J. S. (2022). Hybrid cell line development system utilizing site-specific integration and methotrexate-mediated gene amplification in Chinese hamster ovary cells. Frontiers in Bioengineering and Biotechnology, 10, 977193.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-5) in Immunoblot

MDA-MB-468 cells were treated with PYR or MTX for 24 h and analyzed by immunoblotting with the indicated antibodies. ...View More

Heppler, L. N., Attarha, S., Persaud, R., Brown, J. I., Wang, P., Petrova, B., ... & Frank, D. A. (2022). The antimicrobial drug pyrimethamine inhibits STAT3 transcriptional activity by targeting the enzyme dihydrofolate reductase. Journal of Biological Chemistry, 298(2), 101531.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-6) in WB

Western blot analysis for DHFR expression in HUVECs after silencing DHFR by using 100 pmol siRNA over 48 h. The expression level of DHFR was quantified densitometrically. ...View More

Lee, I., Piao, S., Kim, S., Nagar, H., Choi, S. J., Jeon, B. H., ... & Kim, C. S. (2021). CRIF1 deficiency increased homocysteine production by disrupting dihydrofolate reductase expression in vascular endothelial cells. Antioxidants, 10(11), 1645.

Mouse Anti-DHFR Recombinant Antibody (D0823) (V2LY-0425-LY271-7) in IF

Co-localization of DHFR/SHMT1, SHMT1/TYMS and TYMS/DHFR in asynchronous A549 cells. Co-localization is evident both in the cytosol (tubulin alpha, cyan) and in the nucleus (DAPI, blue) for all the three couples. The original red fluorescence signal of tubulin was changed to cyan for better visualization of the merged images. The merge of green, red and cyan pixels yields white pixels. ...View More

Spizzichino, S., Boi, D., Boumis, G., Lucchi, R., Liberati, F. R., Capelli, D., ... & Cutruzzola, F. (2022). Cytosolic localization and in vitro assembly of human de novo thymidylate synthesis complex. The FEBS Journal, 289(6), 1625-1649.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D0823

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 125-165 near the C-terminus of human DHFR.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

dihydrofolate reductase

Entrez Gene ID

Human	1719
Mouse	13361
Rat	24312

UniProt ID

Human	P00374
Mouse	P00375
Rat	Q920D2

Function

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Biological Process

Axon regeneration Source: BHF-UCL
Dihydrofolate metabolic process Source: BHF-UCL
Folic acid metabolic process Source: BHF-UCL
Glycine biosynthetic process Source: InterPro
Negative regulation of translation Source: CAFA
One-carbon metabolic process Source: UniProtKB-KW
Positive regulation of nitric-oxide synthase activity Source: BHF-UCL
Regulation of removal of superoxide radicals Source: BHF-UCL
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: Reactome
Response to methotrexate Source: BHF-UCL
Tetrahydrobiopterin biosynthetic process Source: BHF-UCL
Tetrahydrofolate biosynthetic process Source: BHF-UCL
Tetrahydrofolate metabolic process Source: UniProtKB

Cellular Location

Cytoplasm; Mitochondrion

Involvement in disease

Megaloblastic anemia due to dihydrofolate reductase deficiency (DHFRD):
An inborn error of metabolism, characterized by megaloblastic anemia and/or pancytopenia, severe cerebral folate deficiency, and cerebral tetrahydrobiopterin deficiency. Clinical features include variable neurologic symptoms, ranging from severe developmental delay and generalized seizures in infancy, to childhood absence epilepsy with learning difficulties, to lack of symptoms.

Huang, K., Narumi, T., Zhang, Y., Li, Q., Murugesan, P., Wu, Y., ... & Cai, H. (2021). Targeting MicroRNA-192-5p, a downstream effector of NOXs (NADPH oxidases), reverses endothelial DHFR (dihydrofolate reductase) deficiency to attenuate abdominal aortic aneurysm formation. Hypertension, 78(2), 282-293.

Ammar, Y. A., Abd El-Hafez, S. M., Hessein, S. A., Ali, A. M., Askar, A. A., & Ragab, A. (2021). One-pot strategy for thiazole tethered 7-ethoxy quinoline hybrids: Synthesis and potential antimicrobial agents as dihydrofolate reductase (DHFR) inhibitors with molecular docking study. Journal of Molecular Structure, 1242, 130748.

Wróbel, A., Baradyn, M., Ratkiewicz, A., & Drozdowska, D. (2021). Synthesis, biological activity, and molecular dynamics study of novel series of a trimethoprim analogs as multi-targeted compounds: Dihydrofolate reductase (DHFR) inhibitors and DNA-binding agents. International journal of molecular sciences, 22(7), 3685.

He, J., Qiao, W., An, Q., Yang, T., & Luo, Y. (2020). Dihydrofolate reductase inhibitors for use as antimicrobial agents. European Journal of Medicinal Chemistry, 195, 112268.

Li, H., Li, Q., Zhang, Y., Liu, W., Gu, B., Narumi, T., ... & Cai, H. (2019). Novel treatment of hypertension by specifically targeting E2F for restoration of endothelial dihydrofolate reductase and eNOS function under oxidative stress. Hypertension, 73(1), 179-189.

Teixeira, B. V. F., Teles, A. L. B., Silva, S. G. D., Brito, C. C. B., Freitas, H. F. D., Pires, A. B. L., ... & Castilho, M. S. (2019). Dual and selective inhibitors of pteridine reductase 1 (PTR1) and dihydrofolate reductase-thymidylate synthase (DHFR-TS) from Leishmania chagasi. Journal of enzyme inhibition and medicinal chemistry, 34(1), 1439-1450.

Hussein, E. M., Al-Rooqi, M. M., Abd El-Galil, S. M., & Ahmed, S. A. (2019). Design, synthesis, and biological evaluation of novel N 4-substituted sulfonamides: acetamides derivatives as dihydrofolate reductase (DHFR) inhibitors. BMC chemistry, 13(1), 1-18.

Francesconi, V., Giovannini, L., Santucci, M., Cichero, E., Costi, M. P., Naesens, L., ... & Tonelli, M. (2018). Synthesis, biological evaluation and molecular modeling of novel azaspiro dihydrotriazines as influenza virus inhibitors targeting the host factor dihydrofolate reductase (DHFR). European journal of medicinal chemistry, 155, 229-243.

Nakano, M., Fukami, T., Gotoh, S., & Nakajima, M. (2017). A-to-I RNA editing up-regulates human dihydrofolate reductase in breast cancer. Journal of Biological Chemistry, 292(12), 4873-4884.

Tonelli, M., Naesens, L., Gazzarrini, S., Santucci, M., Cichero, E., Tasso, B., ... & Loddo, R. (2017). Host dihydrofolate reductase (DHFR)-directed cycloguanil analogues endowed with activity against influenza virus and respiratory syncytial virus. European journal of medicinal chemistry, 135, 467-478.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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