Rabbit Anti-DNAJA1 Recombinant Antibody (
23C6) (V2LY-0725-LY1288)

Name: Rabbit Anti-DNAJA1 Recombinant Antibody (23C6)
SKU: V2LY-0725-LY1288
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-DNAJA1 Recombinant Antibody (23C6) (V2LY-0725-LY1288) in WB

Western Blot
Positive WB detected in: U251 whole cell lysate, Ntera-2 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate
All lanes: DNAJA1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45, 38 kDa
Observed band size: 45-55 kDa

Rabbit Anti-DNAJA1 Recombinant Antibody (23C6) (V2LY-0725-LY1288) in IF

Immunofluorescence staining of A549 cell with V2LY-0725-LY1288 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 550-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Rabbit Anti-DNAJA1 Recombinant Antibody (23C6) (V2LY-0725-LY1288) in FC

Overlay Peak curve showing A549 cells stained with V2LY-0725-LY1288 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4°C.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

23C6

Application

ELISA, WB, IF, FC

Immunogen

A synthesized peptide derived from human DNAJA1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IF	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DnaJ (Hsp40) homolog, subfamily A, member 1

Entrez Gene ID

3301

UniProt ID

P31689

Function

Co-chaperone for HSPA8/Hsc70 (PubMed:10816573).

Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877).

Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510).

Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).

Biological Process

Androgen receptor signaling pathway Source: Ensembl
DNA damage response, detection of DNA damage Source: Ensembl
Flagellated sperm motility Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
Negative regulation of JUN kinase activity Source: UniProtKB
Negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of protein ubiquitination Source: ParkinsonsUK-UCL
Positive regulation of apoptotic process Source: UniProtKB
Protein folding Source: ProtInc
Protein localization to mitochondrion Source: UniProtKB
Regulation of protein transport Source: UniProtKB
Response to heat Source: InterPro
Response to unfolded protein Source: ProtInc
Spermatogenesis Source: Ensembl
Toxin transport Source: Ensembl

Cellular Location

Nucleus; Mitochondrion; Microsome; Cytoplasm; Membrane; Perinuclear region. Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.

Kaida, A., Yamamoto, S., Parrales, A., Young, E. D., Ranjan, A., Alalem, M. A., ... & Iwakuma, T. (2021). DNAJA1 promotes cancer metastasis through interaction with mutant p53. Oncogene, 40(31), 5013-5025.

Tong, X., Xu, D., Mishra, R. K., Jones, R. D., Sun, L., Schiltz, G. E., ... & Yang, G. Y. (2021). Identification of a druggable protein–protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1. Journal of Biological Chemistry, 296.

Roth, H. E., Bhinderwala, F., Franco, R., Zhou, Y., & Powers, R. (2021). DNAJA1 Dysregulates Metabolism Promoting an Antiapoptotic Phenotype in Pancreatic Ductal Adenocarcinoma. Journal of proteome research, 20(8), 3925-3939.

Akber, U., Jo, H., Jeon, S., Yang, S. J., Bong, S., Lim, S., ... & Park, C. S. (2021). Cereblon regulates the proteotoxicity of tau by tuning the chaperone activity of DNAJA1. Journal of Neuroscience, 41(24), 5138-5156.

Yang, S., Ren, X., Liang, Y., Yan, Y., Zhou, Y., Hu, J., ... & Liang, L. (2020). KNK437 restricts the growth and metastasis of colorectal cancer via targeting DNAJA1/CDC45 axis. Oncogene, 39(2), 249-261.

Rodríguez-González, C., Lin, S., Arkan, S., & Hansen, C. (2020). Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation. Scientific reports, 10(1), 1-9.

Blackman, J. S., Knighton, L. E., Takakuwa, J. E., Calderwood, S. K., & Truman, A. W. (2020). Chemogenomic screening identifies the Hsp70 co-chaperone DNAJA1 as a hub for anticancer drug resistance. Scientific reports, 10(1), 1-10.

Xu, D., Tong, X., Sun, L., Li, H., Jones, R. D., Liao, J., & Yang, G. Y. (2019). Inhibition of mutant Kras and p53‐driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53. Molecular carcinogenesis, 58(11), 2052-2064.

Wang, C., Yang, Z. Z., Guo, F. H., Shi, S., Han, X. S., Zeng, A., ... & Jing, Q. (2019). Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. Journal of Biological Chemistry, 294(25), 9873-9887.

Malheiros, J. M., Enríquez-Valencia, C. E., da Silva Duran, B. O., de Paula, T. G., Curi, R. A., de Vasconcelos Silva, J. A. I., ... & Chardulo, L. A. L. (2018). Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Meat science, 138, 49-52.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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