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Mouse Anti-DNAJA1 Recombinant Antibody (KA2A5.6) (CBMAB-D1255-YC)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
KA2A5.6
Application
FC, IF, IHC-P, IP, WB
Specificity
Human
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
DnaJ (Hsp40) homolog, subfamily A, member 1
Introduction
DNAJA1 belongs to the DnaJ family of proteins, which act as heat shock protein 70 cochaperones. Heat shock proteins facilitate protein folding, trafficking, prevention of aggregation, and proteolytic degradation. Members of this family are characterized by a highly conserved N-terminal J domain, a glycine/phenylalanine-rich region, four CxxCxGxG zinc finger repeats, and a C-terminal substrate-binding domain. The J domain mediates the interaction with heat shock protein 70 to recruit substrates and regulate ATP hydrolysis activity. In humans, this gene has been implicated in positive regulation of virus replication through co-option by the influenza A virus.
Entrez Gene ID
3301
UniProt ID
P31689
Alternative Names
DnaJ Heat Shock Protein Family (Hsp40) Member A1; Neural Precursor Cell Expressed, Developmentally Down-Regulated 7; DnaJ (Hsp40) Homolog, Subfamily A, Member 1; Heat Shock 40 KDa Protein 4; DnaJ Protein Homolog 2; Heat Shock Protein J2; Human DnaJ Protein 2; HDJ-2; HSJ-2; HSPF4;
Function
Co-chaperone for HSPA8/Hsc70 (PubMed:10816573).

Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877).

Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510).

Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
Biological Process
Androgen receptor signaling pathway Source: Ensembl
DNA damage response, detection of DNA damage Source: Ensembl
Flagellated sperm motility Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
Negative regulation of JUN kinase activity Source: UniProtKB
Negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of protein ubiquitination Source: ParkinsonsUK-UCL
Positive regulation of apoptotic process Source: UniProtKB
Protein folding Source: ProtInc
Protein localization to mitochondrion Source: UniProtKB
Regulation of protein transport Source: UniProtKB
Response to heat Source: InterPro
Response to unfolded protein Source: ProtInc
Spermatogenesis Source: Ensembl
Toxin transport Source: Ensembl
Cellular Location
Nucleus; Mitochondrion; Microsome; Cytoplasm; Membrane; Perinuclear region. Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.

Kaida, A., Yamamoto, S., Parrales, A., Young, E. D., Ranjan, A., Alalem, M. A., ... & Iwakuma, T. (2021). DNAJA1 promotes cancer metastasis through interaction with mutant p53. Oncogene, 40(31), 5013-5025.

Tong, X., Xu, D., Mishra, R. K., Jones, R. D., Sun, L., Schiltz, G. E., ... & Yang, G. Y. (2021). Identification of a druggable protein–protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1. Journal of Biological Chemistry, 296.

Roth, H. E., Bhinderwala, F., Franco, R., Zhou, Y., & Powers, R. (2021). DNAJA1 Dysregulates Metabolism Promoting an Antiapoptotic Phenotype in Pancreatic Ductal Adenocarcinoma. Journal of proteome research, 20(8), 3925-3939.

Akber, U., Jo, H., Jeon, S., Yang, S. J., Bong, S., Lim, S., ... & Park, C. S. (2021). Cereblon regulates the proteotoxicity of tau by tuning the chaperone activity of DNAJA1. Journal of Neuroscience, 41(24), 5138-5156.

Yang, S., Ren, X., Liang, Y., Yan, Y., Zhou, Y., Hu, J., ... & Liang, L. (2020). KNK437 restricts the growth and metastasis of colorectal cancer via targeting DNAJA1/CDC45 axis. Oncogene, 39(2), 249-261.

Rodríguez-González, C., Lin, S., Arkan, S., & Hansen, C. (2020). Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation. Scientific reports, 10(1), 1-9.

Blackman, J. S., Knighton, L. E., Takakuwa, J. E., Calderwood, S. K., & Truman, A. W. (2020). Chemogenomic screening identifies the Hsp70 co-chaperone DNAJA1 as a hub for anticancer drug resistance. Scientific reports, 10(1), 1-10.

Xu, D., Tong, X., Sun, L., Li, H., Jones, R. D., Liao, J., & Yang, G. Y. (2019). Inhibition of mutant Kras and p53‐driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53. Molecular carcinogenesis, 58(11), 2052-2064.

Wang, C., Yang, Z. Z., Guo, F. H., Shi, S., Han, X. S., Zeng, A., ... & Jing, Q. (2019). Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. Journal of Biological Chemistry, 294(25), 9873-9887.

Malheiros, J. M., Enríquez-Valencia, C. E., da Silva Duran, B. O., de Paula, T. G., Curi, R. A., de Vasconcelos Silva, J. A. I., ... & Chardulo, L. A. L. (2018). Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Meat science, 138, 49-52.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-DNAJA1 Recombinant Antibody (D1255) (CAT#: V2LY-0425-LY610)

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For research use only. Not intended for any clinical use.

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