Sign in or Register   Sign in or Register
  |  

Mouse Anti-DNAJA1 Recombinant Antibody (4F2) (CBMAB-A2301-LY)

The product is antibody recognizes DNAJA1. The antibody 4F2 immunoassay techniques such as: ELISA.
See all DNAJA1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
4F2
Antibody Isotype
IgG2a, κ
Application
ELISA

Basic Information

Immunogen
DNAJA1 (AAH08182, 1 a.a. ~ 397 a.a) full-length recombinant protein with GST-pstS1 tag.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
DnaJ (Hsp40) homolog, subfamily A, member 1
Entrez Gene ID
3301
UniProt ID
P31689
Alternative Names
DJ-2; DjA1; HDJ2; HSDJ; HSJ2; HSPF4; hDJ-2
Function
Co-chaperone for HSPA8/Hsc70 (PubMed:10816573).

Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (PubMed:24318877).

Plays a role in protein transport into mitochondria via its role as co-chaperone. Functions as co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis (PubMed:14752510).

Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV (PubMed:24512202).
Biological Process
Androgen receptor signaling pathway Source: Ensembl
DNA damage response, detection of DNA damage Source: Ensembl
Flagellated sperm motility Source: Ensembl
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of establishment of protein localization to mitochondrion Source: ParkinsonsUK-UCL
Negative regulation of JUN kinase activity Source: UniProtKB
Negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
Negative regulation of protein ubiquitination Source: ParkinsonsUK-UCL
Positive regulation of apoptotic process Source: UniProtKB
Protein folding Source: ProtInc
Protein localization to mitochondrion Source: UniProtKB
Regulation of protein transport Source: UniProtKB
Response to heat Source: InterPro
Response to unfolded protein Source: ProtInc
Spermatogenesis Source: Ensembl
Toxin transport Source: Ensembl
Cellular Location
Nucleus; Mitochondrion; Microsome; Cytoplasm; Membrane; Perinuclear region. Primarily associated with microsomes. A minor proportion is associated with mitochondria (By similarity). Primarily cytoplasmic. A minor proportion is associated with nuclei.

Kaida, A., Yamamoto, S., Parrales, A., Young, E. D., Ranjan, A., Alalem, M. A., ... & Iwakuma, T. (2021). DNAJA1 promotes cancer metastasis through interaction with mutant p53. Oncogene, 40(31), 5013-5025.

Tong, X., Xu, D., Mishra, R. K., Jones, R. D., Sun, L., Schiltz, G. E., ... & Yang, G. Y. (2021). Identification of a druggable protein–protein interaction site between mutant p53 and its stabilizing chaperone DNAJA1. Journal of Biological Chemistry, 296.

Roth, H. E., Bhinderwala, F., Franco, R., Zhou, Y., & Powers, R. (2021). DNAJA1 Dysregulates Metabolism Promoting an Antiapoptotic Phenotype in Pancreatic Ductal Adenocarcinoma. Journal of proteome research, 20(8), 3925-3939.

Akber, U., Jo, H., Jeon, S., Yang, S. J., Bong, S., Lim, S., ... & Park, C. S. (2021). Cereblon regulates the proteotoxicity of tau by tuning the chaperone activity of DNAJA1. Journal of Neuroscience, 41(24), 5138-5156.

Yang, S., Ren, X., Liang, Y., Yan, Y., Zhou, Y., Hu, J., ... & Liang, L. (2020). KNK437 restricts the growth and metastasis of colorectal cancer via targeting DNAJA1/CDC45 axis. Oncogene, 39(2), 249-261.

Rodríguez-González, C., Lin, S., Arkan, S., & Hansen, C. (2020). Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation. Scientific reports, 10(1), 1-9.

Blackman, J. S., Knighton, L. E., Takakuwa, J. E., Calderwood, S. K., & Truman, A. W. (2020). Chemogenomic screening identifies the Hsp70 co-chaperone DNAJA1 as a hub for anticancer drug resistance. Scientific reports, 10(1), 1-10.

Xu, D., Tong, X., Sun, L., Li, H., Jones, R. D., Liao, J., & Yang, G. Y. (2019). Inhibition of mutant Kras and p53‐driven pancreatic carcinogenesis by atorvastatin: Mainly via targeting of the farnesylated DNAJA1 in chaperoning mutant p53. Molecular carcinogenesis, 58(11), 2052-2064.

Wang, C., Yang, Z. Z., Guo, F. H., Shi, S., Han, X. S., Zeng, A., ... & Jing, Q. (2019). Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea. Journal of Biological Chemistry, 294(25), 9873-9887.

Malheiros, J. M., Enríquez-Valencia, C. E., da Silva Duran, B. O., de Paula, T. G., Curi, R. A., de Vasconcelos Silva, J. A. I., ... & Chardulo, L. A. L. (2018). Association of CAST2, HSP90AA1, DNAJA1 and HSPB1 genes with meat tenderness in Nellore cattle. Meat science, 138, 49-52.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DNAJA1 Recombinant Antibody (4F2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare