Mouse Anti-DNAJC2 Recombinant Antibody (D1312) (V2LY-0425-LY650)

Name: Mouse Anti-DNAJC2 Recombinant Antibody (D1312)
SKU: V2LY-0425-LY650
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D1312

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 10-29 at the N-terminus of human Zuotin-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgM, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DnaJ Heat Shock Protein Family (Hsp40) Member C2

Entrez Gene ID

Human	27000
Mouse	22791
Rat	116456

UniProt ID

Human	Q99543
Mouse	P54103
Rat	Q7TQ20

Function

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation.

Biological Process

de novo' cotranslational protein folding Source: UniProtKB
Chromatin organization Source: UniProtKB-KW
DNA replication Source: Ensembl
Negative regulation of DNA biosynthetic process Source: Ensembl
Positive regulation of transcription, DNA-templated Source: UniProtKB
Regulation of cellular response to heat Source: Reactome
Regulation of translational fidelity Source: InterPro

Cellular Location

Nucleus; Cytosol

PTM

Phosphorylated in M (mitotic) phase.

Liu, H., Li, J., Zhao, H., Liu, X., & Ye, X. (2021). DNAJC2 is reversely regulated by miR‑627‑3p, promoting the proliferation of colorectal cancer. Molecular Medicine Reports, 24(2), 1-10.

Yoshida, Y., Zhang, X. M., Wang, H., Machida, T., Mine, S., Kobayashi, E., ... & Hiwasa, T. (2020). Elevated levels of autoantibodies against DNAJC2 in sera of patients with atherosclerotic diseases. Heliyon, 6(8), e04661.

Helary, L., Castille, J., Passet, B., Vaiman, A., Beauvallet, C., Jaffrezic, F., ... & Duchesne, A. (2019). DNAJC2 is required for mouse early embryonic development. Biochemical and biophysical research communications, 516(1), 258-263.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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