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Rabbit Anti-DOCK1 Recombinant Antibody (EG984) (CBMAB-EN1166-LY)

The product is antibody recognizes DOCK1. The antibody EG984 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:40000.
See all DOCK1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG984
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human DOCK1.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Dedicator Of Cytokinesis 1
Introduction
This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
Entrez Gene ID
Human1793
Mouse330662
UniProt ID
HumanQ14185
MouseQ8BUR4
Alternative Names
Dedicator Of Cytokinesis 1; 180 KDa Protein Downstream Of CRK; DOwnstream Of CrK; DOCK180; Dedicator Of Cytokinesis Protein 1; Dedicator Of Cyto-Kinesis 1; Ced5;
Function
Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829).

Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed:8657152).
Biological Process
Apoptotic process Source: ProtInc
Integrin-mediated signaling pathway Source: ProtInc
Phagocytosis, engulfment Source: ProtInc
Positive regulation of epithelial cell migration Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: ProtInc
Cellular Location
Cytoplasm; Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate.

Yang, X., Wang, Y., Pang, S., Li, X., Wang, P., Ma, R., ... & Song, C. (2021). LINC00665 promotes the progression of acute myeloid leukemia by regulating the miR-4458/DOCK1 pathway. Scientific reports, 11(1), 1-16.

Cui, W., & Xue, J. (2020). Circular RNA DOCK1 downregulates microRNA‐124 to induce the growth of human thyroid cancer cell lines. Biofactors, 46(4), 591-599.

Liu, P., Li, X., Guo, X., Chen, J., Li, C., Chen, M., ... & Zu, X. (2019). Circular RNA DOCK1 promotes bladder carcinoma progression via modulating circDOCK1/hsa‐miR‐132‐3p/Sox5 signalling pathway. Cell proliferation, 52(4), e12614.

Chiang, S. K., Chang, W. C., Chen, S. E., & Chang, L. C. (2019). DOCK1 regulates growth and motility through the RRP1B-claudin-1 pathway in claudin-low breast cancer cells. Cancers, 11(11), 1762.

Tomino, T., Tajiri, H., Tatsuguchi, T., Shirai, T., Oisaki, K., Matsunaga, S., ... & Uruno, T. (2018). DOCK1 inhibition suppresses cancer cell invasion and macropinocytosis induced by self-activating Rac1P29S mutation. Biochemical and biophysical research communications, 497(1), 298-304.

Liang, Y., Wang, S., & Zhang, Y. (2018). Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple‑negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncology Letters, 16(3), 3481-3488.

Cunningham, R. L., Herbert, A. L., Harty, B. L., Ackerman, S. D., & Monk, K. R. (2018). Mutations in dock1 disrupt early Schwann cell development. Neural development, 13(1), 1-15.

Zhang, B., Li, H., Yin, C., Sun, X., Zheng, S., Zhang, C., ... & Lu, S. (2017). Dock1 promotes the mesenchymal transition of glioma and is modulated by MiR‐31. Neuropathology and Applied Neurobiology, 43(5), 419-432.

Tajiri, H., Uruno, T., Shirai, T., Takaya, D., Matsunaga, S., Setoyama, D., ... & Fukui, Y. (2017). Targeting Ras-driven cancer cell survival and invasion through selective inhibition of DOCK1. Cell reports, 19(5), 969-980.

Lee, S. H., Chiu, Y. C., Li, Y. H., Lin, C. C., Hou, H. A., Chou, W. C., & Tien, H. F. (2017). High expression of dedicator of cytokinesis 1 (DOCK1) confers poor prognosis in acute myeloid leukemia. Oncotarget, 8(42), 72250.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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