Mouse Dock1 ELISA Kit (V2LY-0626-LY2267)

Name: Mouse Dock1 ELISA Kit
SKU: V2LY-0626-LY2267
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.014 ng/mL

Detection Range

0.03-9 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Mouse

Assay Type

Sandwich

Reactivity

Mouse

Assay Time

1.5 h

Molecule Mass

215.1 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Dedicator Of Cytokinesis 1

Function

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Along with DOCK1, mediates CRK/CRKL regulation of epithelial and endothelial cell spreading and migration on type IV collagen (PubMed:19004829).

Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1 (PubMed:8657152).

Biological Process

Apoptotic process Source: ProtInc
Integrin-mediated signaling pathway Source: ProtInc
Phagocytosis, engulfment Source: ProtInc
Positive regulation of epithelial cell migration Source: UniProtKB
Positive regulation of substrate adhesion-dependent cell spreading Source: UniProtKB
Signal transduction Source: ProtInc
Small GTPase mediated signal transduction Source: ProtInc

Cellular Location

Cytoplasm; Membrane. Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate.

Yang, X., Wang, Y., Pang, S., Li, X., Wang, P., Ma, R., ... & Song, C. (2021). LINC00665 promotes the progression of acute myeloid leukemia by regulating the miR-4458/DOCK1 pathway. Scientific reports, 11(1), 1-16.

Cui, W., & Xue, J. (2020). Circular RNA DOCK1 downregulates microRNA‐124 to induce the growth of human thyroid cancer cell lines. Biofactors, 46(4), 591-599.

Liu, P., Li, X., Guo, X., Chen, J., Li, C., Chen, M., ... & Zu, X. (2019). Circular RNA DOCK1 promotes bladder carcinoma progression via modulating circDOCK1/hsa‐miR‐132‐3p/Sox5 signalling pathway. Cell proliferation, 52(4), e12614.

Chiang, S. K., Chang, W. C., Chen, S. E., & Chang, L. C. (2019). DOCK1 regulates growth and motility through the RRP1B-claudin-1 pathway in claudin-low breast cancer cells. Cancers, 11(11), 1762.

Tomino, T., Tajiri, H., Tatsuguchi, T., Shirai, T., Oisaki, K., Matsunaga, S., ... & Uruno, T. (2018). DOCK1 inhibition suppresses cancer cell invasion and macropinocytosis induced by self-activating Rac1P29S mutation. Biochemical and biophysical research communications, 497(1), 298-304.

Liang, Y., Wang, S., & Zhang, Y. (2018). Downregulation of Dock1 and Elmo1 suppresses the migration and invasion of triple‑negative breast cancer epithelial cells through the RhoA/Rac1 pathway. Oncology Letters, 16(3), 3481-3488.

Cunningham, R. L., Herbert, A. L., Harty, B. L., Ackerman, S. D., & Monk, K. R. (2018). Mutations in dock1 disrupt early Schwann cell development. Neural development, 13(1), 1-15.

Zhang, B., Li, H., Yin, C., Sun, X., Zheng, S., Zhang, C., ... & Lu, S. (2017). Dock1 promotes the mesenchymal transition of glioma and is modulated by MiR‐31. Neuropathology and Applied Neurobiology, 43(5), 419-432.

Tajiri, H., Uruno, T., Shirai, T., Takaya, D., Matsunaga, S., Setoyama, D., ... & Fukui, Y. (2017). Targeting Ras-driven cancer cell survival and invasion through selective inhibition of DOCK1. Cell reports, 19(5), 969-980.

Lee, S. H., Chiu, Y. C., Li, Y. H., Lin, C. C., Hou, H. A., Chou, W. C., & Tien, H. F. (2017). High expression of dedicator of cytokinesis 1 (DOCK1) confers poor prognosis in acute myeloid leukemia. Oncotarget, 8(42), 72250.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human DOCK1 ELISA Kit (CAT#: V2LY-0626-LY4483)

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Rabbit Anti-DOCK1 Recombinant Antibody (H.398.0) (CAT#: CBMAB-D1473-YC)

Mouse Anti-DOCK1 Recombinant Antibody (3D10) (CAT#: V2LY-0425-LY765)

Human DOCK1 ELISA Kit (CAT#: V2LY-0626-LY4483)

Mouse Anti-DOCK1 Recombinant Antibody (EG984) (CAT#: V2LY-0425-LY766)

Mouse Anti-DOCK1 Recombinant Antibody (D1473) (CAT#: V2LY-0425-LY764)

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Isotype control

For research use only. Not intended for any clinical use.

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