Mouse Anti-DOK3 Recombinant Antibody (EG988) (V2LY-0425-LY792)

Name: Mouse Anti-DOK3 Recombinant Antibody (EG988)
SKU: V2LY-0425-LY792
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG988

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 231-444 mapping at the C-terminus of mouse Dok-3.

Host Species

Mouse

Specificity

Mouse

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Docking Protein 3

Entrez Gene ID

27261

UniProt ID

Q9QZK7

Function

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function (By similarity).

Cellular Location

Cytoplasm; Cell membrane

PTM

Constitutively tyrosine-phosphorylated.
On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL1 kinase (By similarity).

Zhang, Y., Zhang, S., Wang, Y., Yang, Z., Chen, Z., Wen, N., ... & Cai, L. (2021). ULK1 Suppresses Osteoclast Differentiation and Bone Resorption via Inhibiting Syk-JNK through DOK3. Oxidative medicine and cellular longevity, 2021.

Liu, X., Chen, F., & Li, W. (2020). Elevated expression of DOK3 indicates high suppressive immune cell infiltration and unfavorable prognosis of gliomas. International Immunopharmacology, 83, 106400.

Cai, X., Xing, J., Long, C. L., Peng, Q., & Humphrey, M. B. (2017). DOK3 modulates bone remodeling by negatively regulating osteoclastogenesis and positively regulating osteoblastogenesis. Journal of Bone and Mineral Research, 32(11), 2207-2218.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-DOK3 Recombinant Antibody (EG988) (CAT#: CBMAB-EN1170-LY)

Related Products

Mouse Anti-DOK3 Recombinant Antibody (CBYCD-378) (CAT#: V2LY-0425-LY791)

Mouse Anti-DOK3 Recombinant Antibody (CBYCD-378) (CAT#: CBMAB-D1506-YC)

Rabbit Anti-DOK3 Recombinant Antibody (EG988) (CAT#: CBMAB-EN1170-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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