Mouse Anti-DPH2 Recombinant Antibody (1A12) (CBMAB-D1551-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DPH2 Homolog (DPH2). The antibody can be used for immunoassay techniques, such as IHC-P, WB.
See all DPH2 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1A12

Antibody Isotype

IgG1

Application

IHC-P, WB

Basic Information

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DPH2 homolog (S. cerevisiae)

Introduction

DPH2 is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation.

Entrez Gene ID

1802

UniProt ID

Q9BQC3

Alternative Names

DPH2 Homolog; S-Adenosyl-L-Methionine:L-Histidine 3-Amino-3-Carboxypropyltransferase 2; Diphtheria Toxin Resistance Protein 2; Diphthamide Biosynthesis Protein 2; DPH2L2; Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2 (S. Cerevisiae); Diptheria Toxin Resistance Protein Required For Diphthamide Biosynthesis-Like 2; 2-(3-Amino-3-Carboxypropyl)Histidine Synthase Subunit 2;

Function

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).

Biological Process

Peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central

Cellular Location

Cytosol

Topology

Extracellular: 30-448
Helical: 449-469
Cytoplasmic: 470-517

References

Feng, J., Shaik, S., & Wang, B. (2021). Spin‐Regulated Electron Transfer and Exchange‐Enhanced Reactivity in Fe4S4‐Mediated Redox Reaction of the Dph2 Enzyme During the Biosynthesis of Diphthamide. Angewandte Chemie, 133(37), 20593-20599.

Zhang, Y., Su, D., Dzikovski, B., Majer, S. H., Coleman, R., Chandrasekaran, S., ... & Lin, H. (2021). Dph3 Enables Aerobic Diphthamide Biosynthesis by Donating One Iron Atom to Transform a [3Fe–4S] to a [4Fe–4S] Cluster in Dph1–Dph2. Journal of the American Chemical Society, 143(25), 9314-9319.

Dong, M., Dando, E. E., Kotliar, I., Su, X., Dzikovski, B., Freed, J. H., & Lin, H. (2019). The asymmetric function of Dph1–Dph2 heterodimer in diphthamide biosynthesis. JBIC Journal of Biological Inorganic Chemistry, 24(6), 777-782.

Fenwick, M. K., Dong, M., Lin, H., & Ealick, S. E. (2019). The crystal structure of Dph2 in complex with elongation factor 2 reveals the structural basis for the first step of diphthamide biosynthesis. Biochemistry, 58(43), 4343-4351.

Dong, M., Kathiresan, V., Fenwick, M. K., Torelli, A. T., Zhang, Y., Caranto, J. D., ... & Lin, H. (2018). Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science, 359(6381), 1247-1250.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-DPH2 Recombinant Antibody (1A12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-DPH2 Recombinant Antibody (5B10)

Mouse Anti-DPH2 Recombinant Antibody (3A12)

Mouse Anti-DPH2 Recombinant Antibody (2B12)

Mouse Anti-DPH2 Recombinant Antibody (4D9)

Mouse Anti-DPH2 Recombinant Antibody (3F7)

Mouse Anti-DPH2 Recombinant Antibody (4E7)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: