Mouse Anti-DPH2 Recombinant Antibody (2B12) (V2LY-0425-LY823)

Name: Mouse Anti-DPH2 Recombinant Antibody (2B12)
SKU: V2LY-0425-LY823
Rating: 5 (1 reviews)

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Summary

Host Animal

Mouse

Specificity

Human, Rat

Clone

2B12

Antibody Isotype

IgG2b

Application

FC, IHC, WB

Basic Information

Immunogen

Full length human recombinant protein of human DPH2 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:150
WB	1:2,000
FC	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DPH2 homolog (S. cerevisiae)

Entrez Gene ID

1802

UniProt ID

Q9BQC3

Function

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).

Biological Process

Peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central

Cellular Location

Cytosol

Topology

Extracellular: 30-448
Helical: 449-469
Cytoplasmic: 470-517

More Infomation

References

Feng, J., Shaik, S., & Wang, B. (2021). Spin‐Regulated Electron Transfer and Exchange‐Enhanced Reactivity in Fe4S4‐Mediated Redox Reaction of the Dph2 Enzyme During the Biosynthesis of Diphthamide. Angewandte Chemie, 133(37), 20593-20599.

Zhang, Y., Su, D., Dzikovski, B., Majer, S. H., Coleman, R., Chandrasekaran, S., ... & Lin, H. (2021). Dph3 Enables Aerobic Diphthamide Biosynthesis by Donating One Iron Atom to Transform a [3Fe–4S] to a [4Fe–4S] Cluster in Dph1–Dph2. Journal of the American Chemical Society, 143(25), 9314-9319.

Dong, M., Dando, E. E., Kotliar, I., Su, X., Dzikovski, B., Freed, J. H., & Lin, H. (2019). The asymmetric function of Dph1–Dph2 heterodimer in diphthamide biosynthesis. JBIC Journal of Biological Inorganic Chemistry, 24(6), 777-782.

Fenwick, M. K., Dong, M., Lin, H., & Ealick, S. E. (2019). The crystal structure of Dph2 in complex with elongation factor 2 reveals the structural basis for the first step of diphthamide biosynthesis. Biochemistry, 58(43), 4343-4351.

Dong, M., Kathiresan, V., Fenwick, M. K., Torelli, A. T., Zhang, Y., Caranto, J. D., ... & Lin, H. (2018). Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science, 359(6381), 1247-1250.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Alternative Versions

Mouse Anti-DPH2 Recombinant Antibody (2B12)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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