Mouse Anti-DPH2 Recombinant Antibody (1A12) (V2LY-0425-LY822)

Name: Mouse Anti-DPH2 Recombinant Antibody (1A12)
SKU: V2LY-0425-LY822
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

1A12

Application

IHC, WB

Immunogen

Full length human recombinant protein of human DPH2 produced in HEK293T cell.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:150
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

BSA & Glycerol & PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

DPH2 homolog (S. cerevisiae)

Entrez Gene ID

1802

UniProt ID

Q9BQC3

Function

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).

Biological Process

Peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: GO_Central

Cellular Location

Cytosol

Topology

Extracellular: 30-448
Helical: 449-469
Cytoplasmic: 470-517

Feng, J., Shaik, S., & Wang, B. (2021). Spin‐Regulated Electron Transfer and Exchange‐Enhanced Reactivity in Fe4S4‐Mediated Redox Reaction of the Dph2 Enzyme During the Biosynthesis of Diphthamide. Angewandte Chemie, 133(37), 20593-20599.

Zhang, Y., Su, D., Dzikovski, B., Majer, S. H., Coleman, R., Chandrasekaran, S., ... & Lin, H. (2021). Dph3 Enables Aerobic Diphthamide Biosynthesis by Donating One Iron Atom to Transform a [3Fe–4S] to a [4Fe–4S] Cluster in Dph1–Dph2. Journal of the American Chemical Society, 143(25), 9314-9319.

Dong, M., Dando, E. E., Kotliar, I., Su, X., Dzikovski, B., Freed, J. H., & Lin, H. (2019). The asymmetric function of Dph1–Dph2 heterodimer in diphthamide biosynthesis. JBIC Journal of Biological Inorganic Chemistry, 24(6), 777-782.

Fenwick, M. K., Dong, M., Lin, H., & Ealick, S. E. (2019). The crystal structure of Dph2 in complex with elongation factor 2 reveals the structural basis for the first step of diphthamide biosynthesis. Biochemistry, 58(43), 4343-4351.

Dong, M., Kathiresan, V., Fenwick, M. K., Torelli, A. T., Zhang, Y., Caranto, J. D., ... & Lin, H. (2018). Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis. Science, 359(6381), 1247-1250.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Alternative Versions

Mouse Anti-DPH2 Recombinant Antibody (1A12) (CAT#: CBMAB-D1551-YC)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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