Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982)

Name: Mouse Anti-DSG2 Recombinant Antibody (D1769)
SKU: V2LY-0425-LY982
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982-1) in Immunoblot

Samples were prepared and immunoblotted with antibodies to the indicated proteins. ...View More

Koyama-Nasu, R., Takahashi, R., Yanagida, S., Nasu-Nishimura, Y., Oyama, M., Kozuka-Hata, H., ... & Akiyama, T. (2013). The cancer stem cell marker CD133 interacts with plakoglobin and controls desmoglein-2 protein levels. PLoS One, 8(1), e53710.

Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982-2) in IHC

Co-localization of CD133 (red) with desmosomal proteins (green). CCC stem cells were immunostained with antibodies to the indicated proteins. TO-PRO-3 iodide was used for nuclear DNA staining (blue). Scale bars represent 20 µm. ...View More

Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982-3) in Immunoblot

Wild-type, DSG-2 CRISPR-cas9 knockout, and DSG-2 knockout reconstituted with DSG-2 tension sensor A431 cells were lysed and assessed for phosphorylated epidermal growth factor receptor (EGFR) (pY1173). Reconstitution with the DSG-2 tension sensor rescued the loss of phosphorylated EGFR seen in the knockout cells. ...View More

Baddam, S. R., Arsenovic, P. T., Narayanan, V., Duggan, N. R., Mayer, C. R., Newman, S. T., ... & Conway, D. E. (2018). The desmosomal cadherin desmoglein-2 experiences mechanical tension as demonstrated by a FRET-based tension biosensor expressed in living cells. Cells, 7(7), 66.

Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982-4) in WB and IHC

Western immunoblots and immunolabeled myocardium probed for desmoglein-2 (DSG2) showed a complete absence of protein and junctional distribution of DSG2 in Dsg2mut/mut mice compared with WT mice. Scale bar: 10 μm. ...View More

Chelko, S. P., Asimaki, A., Andersen, P., Bedja, D., Amat-Alarcon, N., DeMazumder, D., ... & Judge, D. P. (2016). Central role for GSK3β in the pathogenesis of arrhythmogenic cardiomyopathy. JCI insight, 1(5), e85923.

Mouse Anti-DSG2 Recombinant Antibody (D1769) (V2LY-0425-LY982-5) in IF

Immunofluorescence staining of differentiated human bronchial epithelial cells.
Confocal microscopy immunofluorescence analysis of differentiated human bronchial epithelial cells. Figs A, B, C show XY planes, Figs D, E, F show XZ planes. (A, D) Cells were infected from the apical side with HAdV-B14p1 at a moi of 10 (TCID50/cell), fixated 4 days p.i. and stained for HAdV in green (FITC conjugated antibody) and the desmoglein 2 (DSG2 receptor) in red (dsRed antibody), the nucleus was counterstained in blue (DAPI). (B, E) Differentiated human bronchial epithelial cells stained for DSG2 receptor in red (dsRed) and occludin (OCLN) a tight junction marker in green (FITC), nucleus was counterstained in blue (DAPI). (C, F) Differentiated human bronchial epithelial cells were stained for CAR receptor in red (dsRed) and the tight junction marker OCLN in green (FITC) and the nucleus in blue (DAPI). ...View More

Lam, E., Ramke, M., Warnecke, G., Schrepfer, S., Kopfnagel, V., Dobner, T., & Heim, A. (2015). Effective apical infection of differentiated human bronchial epithelial cells and induction of proinflammatory chemokines by the highly pneumotropic human adenovirus type 14p1. PLoS One, 10(7), e0131201.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D1769

Application

WB, IP, IF

Immunogen

Recombinant Human DSG2 protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Desmoglein 2

Entrez Gene ID

1829

UniProt ID

Q14126

Research Area

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Biological Process

Bundle of His cell-Purkinje myocyte adhesion involved in cell communication Source: BHF-UCL
Cell adhesion Source: UniProtKB
Cell-cell adhesion Source: GO_Central
Desmosome organization Source: BHF-UCL
Homophilic cell adhesion via plasma membrane adhesion molecules Source: BHF-UCL
Purkinje myocyte development Source: BHF-UCL
Regulation of heart rate by cardiac conduction Source: BHF-UCL
Regulation of ventricular cardiac muscle cell action potential Source: BHF-UCL

Cellular Location

Cell membrane; Desmosome

Involvement in disease

Arrhythmogenic right ventricular dysplasia, familial, 10 (ARVD10):
A congenital heart disease characterized by infiltration of adipose and fibrous tissue into the right ventricle and loss of myocardial cells, resulting in ventricular and supraventricular arrhythmias.
Cardiomyopathy, dilated 1BB (CMD1BB):
A disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.

Topology

Extracellular: 50-609
Helical: 610-634
Cytoplasmic: 635-1118

Brodehl, A., Meshkov, A., Myasnikov, R., Kiseleva, A., Kulikova, O., Klauke, B., ... & Milting, H. (2021). Hemi-and homozygous loss-of-function mutations in dsg2 (Desmoglein-2) cause recessive arrhythmogenic cardiomyopathy with an early onset. International journal of molecular sciences, 22(7), 3786.

Sun, R., Ma, C., Wang, W., & Yang, S. (2020). Upregulation of desmoglein 2 and its clinical value in lung adenocarcinoma: a comprehensive analysis by multiple bioinformatics methods. PeerJ, 8, e8420.

Kim, J., Beidler, P., Wang, H., Li, C., Quassab, A., Coles, C., ... & Lieber, A. (2020). Desmoglein-2 as a prognostic and biomarker in ovarian cancer. Cancer Biology & Therapy, 21(12), 1154-1162.

Debus, J. D., Milting, H., Brodehl, A., Kassner, A., Anselmetti, D., Gummert, J., & Gaertner-Rommel, A. (2019). In vitro analysis of arrhythmogenic cardiomyopathy associated desmoglein-2 (DSG2) mutations reveals diverse glycosylation patterns. Journal of molecular and cellular cardiology, 129, 303-313.

Hermida, A., Fressart, V., Hidden‐Lucet, F., Donal, E., Probst, V., Deharo, J. C., ... & Gandjbakhch, E. (2019). High risk of heart failure associated with desmoglein‐2 mutations compared to plakophilin‐2 mutations in arrhythmogenic right ventricular cardiomyopathy/dysplasia. European journal of heart failure, 21(6), 792-800.

Han, C. P., Yu, Y. H., Wang, A. G., Tian, Y., Zhang, H. T., Zheng, Z. M., & Liu, Y. S. (2018). Desmoglein-2 overexpression predicts poor prognosis in hepatocellular carcinoma patients. Eur Rev Med Pharmacol Sci, 22(17), 5481-5489.

Gross, A., Pack, L. A., Schacht, G. M., Kant, S., Ungewiss, H., Meir, M., ... & Strnad, P. (2018). Desmoglein 2, but not desmocollin 2, protects intestinal epithelia from injury. Mucosal immunology, 11(6), 1630-1639.

Hütz, K., Zeiler, J., Sachs, L., Ormanns, S., & Spindler, V. (2017). Loss of desmoglein 2 promotes tumorigenic behavior in pancreatic cancer cells. Molecular Carcinogenesis, 56(8), 1884-1895.

Ungewiß, H., Vielmuth, F., Suzuki, S. T., Maiser, A., Harz, H., Leonhardt, H., ... & Waschke, J. (2017). Desmoglein 2 regulates the intestinal epithelial barrier via p38 mitogen-activated protein kinase. Scientific reports, 7(1), 1-10.

Overmiller, A. M., Pierluissi, J. A., Wermuth, P. J., Sauma, S., Martinez‐Outschoorn, U., Tuluc, M., ... & Mahoney, M. G. (2017). Desmoglein 2 modulates extracellular vesicle release from squamous cell carcinoma keratinocytes. The FASEB Journal, 31(8), 3412-3424.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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