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Rabbit Anti-DUSP1 Recombinant Antibody (PTM025YC) (V2LY-0425-LY1054)


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Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
PTM025YC
Antibody Isotype
IgG
Application
WB, IP, IHC-P, IF, FC

Basic Information

Immunogen
Synthetic peptide within human dusp1 (aa 112-156).
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:500-1:2,000
IP1-2 μg/ml
IF(ICC)1:100
IHC-P1:1,000
FC1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
BSA & Glycerol & TBS
Preservative
Sodium Azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
dual specificity phosphatase 1
Entrez Gene ID
1843
UniProt ID
P28562
Research Area
Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
Biological Process
Cell cycle Source: UniProtKB-KW
Cellular response to chemokine Source: BHF-UCL
Cellular response to hormone stimulus Source: Ensembl
Endoderm formation Source: GO_Central
Inactivation of MAPK activity Source: UniProtKB
Intracellular signal transduction Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Negative regulation of cell adhesion Source: BHF-UCL
Negative regulation of DNA biosynthetic process Source: Ensembl
Negative regulation of ERK1 and ERK2 cascade Source: Ensembl
Negative regulation of MAPK cascade Source: UniProtKB
Negative regulation of MAP kinase activity Source: UniProtKB
Negative regulation of meiotic cell cycle Source: UniProtKB
Negative regulation of monocyte chemotaxis Source: BHF-UCL
Negative regulation of p38MAPK cascade Source: BHF-UCL
Peptidyl-serine dephosphorylation Source: UniProtKB
Peptidyl-threonine dephosphorylation Source: UniProtKB
Peptidyl-tyrosine dephosphorylation Source: UniProtKB
Positive regulation of apoptotic process Source: Ensembl
Protein dephosphorylation Source: GO_Central
Regulation of cell cycle Source: UniProtKB
Regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Response to calcium ion Source: Ensembl
Response to cAMP Source: Ensembl
Response to estradiol Source: Ensembl
Response to glucocorticoid Source: Ensembl
Response to hydrogen peroxide Source: Ensembl
Response to light stimulus Source: Ensembl
Response to retinoic acid Source: Ensembl
Response to testosterone Source: Ensembl
Cellular Location
Nucleus
PTM
Phosphorylation at Ser-359 and Ser-364 by MAPK1/ERK2 and MAPK3/ERK1 reduces its rate of degradation.
More Infomation

Bermúdez-Muñoz, J. M., Celaya, A. M., García-Mato, Á., Muñoz-Espín, D., Rodríguez-de la Rosa, L., Serrano, M., & Varela-Nieto, I. (2021). Dual-specificity phosphatase 1 (DUSP1) has a central role in redox homeostasis and inflammation in the mouse cochlea. Antioxidants, 10(9), 1351.

Maekawa, A., Sawaji, Y., Endo, K., Kusakabe, T., Konishi, T., Tateiwa, T., ... & Yamamoto, K. (2021). Prostaglandin E2 induces dual-specificity phosphatase-1, thereby attenuating inflammatory genes expression in human osteoarthritic synovial fibroblasts. Prostaglandins & Other Lipid Mediators, 154, 106550.

Pan, J., Lin, M., Xu, Z., Xu, M., Zhang, J., Weng, Z., ... & Lin, X. (2021). CDKN2B antisense RNA 1 suppresses tumor growth in human colorectal cancer by targeting MAPK inactivator dual-specificity phosphatase 1. Carcinogenesis, 42(11), 1399-1409.

Hoppstädter, J., & Ammit, A. J. (2019). Role of dual-specificity phosphatase 1 in glucocorticoid-driven anti-inflammatory responses. Frontiers in Immunology, 10, 1446.

Martínez-Martínez, D., Soto, A., Gil-Araujo, B., Gallego, B., Chiloeches, A., & Lasa, M. (2019). Resveratrol promotes apoptosis through the induction of dual specificity phosphatase 1 and sensitizes prostate cancer cells to cisplatin. Food and chemical toxicology, 124, 273-279.

Fu, X. H., Chen, C. Z., Li, S., Han, D. X., Wang, Y. J., Yuan, B., ... & Jiang, H. (2019). Dual-specificity phosphatase 1 regulates cell cycle progression and apoptosis in cumulus cells by affecting mitochondrial function, oxidative stress, and autophagy. American Journal of Physiology-Cell Physiology, 317(6), C1183-C1193.

Gumpena, R., Lountos, G. T., Raran‐Kurussi, S., Tropea, J. E., Cherry, S., & Waugh, D. S. (2018). Crystal structure of the human dual specificity phosphatase 1 catalytic domain. Protein Science, 27(2), 561-567.

Tuglu, M. M., Bostanabad, S. Y., Ozyon, G., Dalkiliç, B., & Gurdal, H. (2018). The role of dual‑specificity phosphatase 1 and protein phosphatase 1 in β2‑adrenergic receptor‑mediated inhibition of extracellular signal regulated kinase 1/2 in triple negative breast cancer cell lines. Molecular Medicine Reports, 17(1), 2033-2043.

Peng, H. Z., Yun, Z., Wang, W., & Ma, B. A. (2017). Dual specificity phosphatase 1 has a protective role in osteoarthritis fibroblast‑like synoviocytes via inhibition of the MAPK signaling pathway. Molecular Medicine Reports, 16(6), 8441-8447.

Zhao, Y., Wang, S., Chu, Z., Dang, Y., Zhu, J., & Su, X. (2017). MicroRNA-101 in the ventrolateral orbital cortex (VLO) modulates depressive-like behaviors in rats and targets dual-specificity phosphatase 1 (DUSP1). Brain research, 1669, 55-62.

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For research use only. Not intended for any clinical use.

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