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Mouse Anti-E2F8 Antibody (1A10) (CBMAB-0326-YC)

Provided herein are mouse monoclonal antibodies against Human E2F8. The antibody clone 1A10 can be used for immunoassay techniques, such as IP and MA.
See all E2F8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A10
Antibody Isotype
IgG2b
Application
IP, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
E2F8
Introduction
E2F8 (E2F transcription factor 8) is a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. E2F8 regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time.
Entrez Gene ID
79733
UniProt ID
A0AVK6
Alternative Names
E2F-8
Research Area
Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.
Biological Process
Cell cycle comprising mitosis without cytokinesis Source: UniProtKB
Cell population proliferation Source: Ensembl
Chorionic trophoblast cell differentiation Source: UniProtKB
Hepatocyte differentiation Source: UniProtKB
Negative regulation of cytokinesis Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Placenta development Source: UniProtKB
Positive regulation of DNA endoreduplication Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Sprouting angiogenesis Source: UniProtKB
Trophoblast giant cell differentiation Source: UniProtKB
Cellular Location
Nucleus

Yang, C. H., Mangiafico, S. P., Waibel, M., Loudovaris, T., Loh, K., Thomas, H. E., ... & Andrikopoulos, S. (2020). E2f8 and Dlg2 genes have independent effects on impaired insulin secretion associated with hyperglycaemia. Diabetologia, 63(7), 1333-1348.

Eoh, K. J., Kim, H. J., Lee, J. W., Kim, L. K., Park, S., Kim, H. S., ... & Koo, P. J. (2020). E2F8 induces cell proliferation and invasion through the epithelial–Mesenchymal transition and notch signaling pathways in ovarian cancer. International journal of molecular sciences, 21(16), 5813.

Lü, Y., Zhang, J., Li, L., Li, S., & Yang, Z. (2020). Carcinogenesis effects of E2F transcription factor 8 (E2F8) in hepatocellular carcinoma outcomes: an integrated bioinformatic report. Bioscience Reports, 40(2).

Iino, K., Mitobe, Y., Ikeda, K., Takayama, K. I., Suzuki, T., Kawabata, H., ... & Inoue, S. (2020). RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8. Cancer science, 111(1), 148-159.

Kim, L. K., Park, S. A., Eoh, K. J., Heo, T. H., Kim, Y. T., & Kim, H. J. (2020). E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer. International journal of biological sciences, 16(2), 320.

Yu, H., Zhang, D., Li, Z., & Wang, M. (2020). E2F transcription factor 8 promotes proliferation and radioresistance in glioblastoma. Pathology-Research and Practice, 216(8), 153030.

Mizuno, M., Miki, R., Moriyama, Y., Ushida, T., Imai, K., Niimi, K., ... & Kotani, T. (2019). The role of E2F8 in the human placenta. Molecular medicine reports, 19(1), 293-301.

Morrell, B. C., Zhang, L., Schütz, L. F., Perego, M. C., Maylem, E. R. S., & Spicer, L. J. (2019). Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells. Molecular and cellular endocrinology, 498, 110572.

Chang, H., Song, J., Wu, J., & Zhang, Y. (2018). E2F transcription factor 8 promotes cell proliferation via CCND1/p21 in esophageal squamous cell carcinoma. OncoTargets and therapy, 11, 8165.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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