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Mouse Anti-E2F8 (AA 1-432) Recombinant Antibody (3E9-2F5) (CBMAB-E0685-FY)

This product is mouse antibody that recognizes E2F8. The antibody 3E9-2F5 can be used for immunoassay techniques such as: ELISA, WB.
See all E2F8 antibodies
Published Data
Fig.1 aPC/CCdh1 targets e2F7 and e2F8 for degradation.
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Fig.2 Western blot analysis of E2F7, E2F8 and HIF1 expression in lysates from HeLa cells.
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Fig.3 The N-terminal 80 amino acids of HIF1a are required for E2F7/8 binding.
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Fig.4 E2F8 mediates cyclin D1 transcription in a dominant-negative manner.
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Fig.5 Regulation of E2F7 and E2F8 during the DNA-damage response.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3E9-2F5
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
Full length recombinant corresponding to aa1-432 from human E2F8 with GST tag. MW of the GST tag alone is 26kD.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2, 1% BSA
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 1-432

Target

Full Name
E2F8
Introduction
This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Entrez Gene ID
79733
UniProt ID
A0AVK6
Alternative Names
E2F Transcription Factor 8; E2F-8; Transcription Factor E2F8; E2F Family Member 8
Research Area
Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.
Biological Process
Cell cycle comprising mitosis without cytokinesis Source: UniProtKB
Cell population proliferation Source: Ensembl
Chorionic trophoblast cell differentiation Source: UniProtKB
Hepatocyte differentiation Source: UniProtKB
Negative regulation of cytokinesis Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Placenta development Source: UniProtKB
Positive regulation of DNA endoreduplication Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Sprouting angiogenesis Source: UniProtKB
Trophoblast giant cell differentiation Source: UniProtKB
Cellular Location
Nucleus

Yang, C. H., Mangiafico, S. P., Waibel, M., Loudovaris, T., Loh, K., Thomas, H. E., ... & Andrikopoulos, S. (2020). E2f8 and Dlg2 genes have independent effects on impaired insulin secretion associated with hyperglycaemia. Diabetologia, 63(7), 1333-1348.

Eoh, K. J., Kim, H. J., Lee, J. W., Kim, L. K., Park, S., Kim, H. S., ... & Koo, P. J. (2020). E2F8 induces cell proliferation and invasion through the epithelial–Mesenchymal transition and notch signaling pathways in ovarian cancer. International journal of molecular sciences, 21(16), 5813.

Lü, Y., Zhang, J., Li, L., Li, S., & Yang, Z. (2020). Carcinogenesis effects of E2F transcription factor 8 (E2F8) in hepatocellular carcinoma outcomes: an integrated bioinformatic report. Bioscience Reports, 40(2).

Iino, K., Mitobe, Y., Ikeda, K., Takayama, K. I., Suzuki, T., Kawabata, H., ... & Inoue, S. (2020). RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8. Cancer science, 111(1), 148-159.

Kim, L. K., Park, S. A., Eoh, K. J., Heo, T. H., Kim, Y. T., & Kim, H. J. (2020). E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer. International journal of biological sciences, 16(2), 320.

Yu, H., Zhang, D., Li, Z., & Wang, M. (2020). E2F transcription factor 8 promotes proliferation and radioresistance in glioblastoma. Pathology-Research and Practice, 216(8), 153030.

Mizuno, M., Miki, R., Moriyama, Y., Ushida, T., Imai, K., Niimi, K., ... & Kotani, T. (2019). The role of E2F8 in the human placenta. Molecular medicine reports, 19(1), 293-301.

Morrell, B. C., Zhang, L., Schütz, L. F., Perego, M. C., Maylem, E. R. S., & Spicer, L. J. (2019). Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells. Molecular and cellular endocrinology, 498, 110572.

Chang, H., Song, J., Wu, J., & Zhang, Y. (2018). E2F transcription factor 8 promotes cell proliferation via CCND1/p21 in esophageal squamous cell carcinoma. OncoTargets and therapy, 11, 8165.

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For research use only. Not intended for any clinical use.

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