Mouse Anti-E2F8 Recombinant Antibody (CBFYE-0380) (V2LY-0425-LY1259)

Name: Mouse Anti-E2F8 Recombinant Antibody (CBFYE-0380)
SKU: V2LY-0425-LY1259
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0380

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 664-764 mapping near the C-terminus of human E2F-8.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, λ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

E2F8

Entrez Gene ID

79733

UniProt ID

A0AVK6

Research Area

Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene.

Biological Process

Cell cycle comprising mitosis without cytokinesis Source: UniProtKB
Cell population proliferation Source: Ensembl
Chorionic trophoblast cell differentiation Source: UniProtKB
Hepatocyte differentiation Source: UniProtKB
Negative regulation of cytokinesis Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Placenta development Source: UniProtKB
Positive regulation of DNA endoreduplication Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Sprouting angiogenesis Source: UniProtKB
Trophoblast giant cell differentiation Source: UniProtKB

Cellular Location

Nucleus

Yang, C. H., Mangiafico, S. P., Waibel, M., Loudovaris, T., Loh, K., Thomas, H. E., ... & Andrikopoulos, S. (2020). E2f8 and Dlg2 genes have independent effects on impaired insulin secretion associated with hyperglycaemia. Diabetologia, 63(7), 1333-1348.

Eoh, K. J., Kim, H. J., Lee, J. W., Kim, L. K., Park, S., Kim, H. S., ... & Koo, P. J. (2020). E2F8 induces cell proliferation and invasion through the epithelial–Mesenchymal transition and notch signaling pathways in ovarian cancer. International journal of molecular sciences, 21(16), 5813.

Lü, Y., Zhang, J., Li, L., Li, S., & Yang, Z. (2020). Carcinogenesis effects of E2F transcription factor 8 (E2F8) in hepatocellular carcinoma outcomes: an integrated bioinformatic report. Bioscience Reports, 40(2).

Iino, K., Mitobe, Y., Ikeda, K., Takayama, K. I., Suzuki, T., Kawabata, H., ... & Inoue, S. (2020). RNA‐binding protein NONO promotes breast cancer proliferation by post‐transcriptional regulation of SKP2 and E2F8. Cancer science, 111(1), 148-159.

Kim, L. K., Park, S. A., Eoh, K. J., Heo, T. H., Kim, Y. T., & Kim, H. J. (2020). E2F8 regulates the proliferation and invasion through epithelial-mesenchymal transition in cervical cancer. International journal of biological sciences, 16(2), 320.

Yu, H., Zhang, D., Li, Z., & Wang, M. (2020). E2F transcription factor 8 promotes proliferation and radioresistance in glioblastoma. Pathology-Research and Practice, 216(8), 153030.

Mizuno, M., Miki, R., Moriyama, Y., Ushida, T., Imai, K., Niimi, K., ... & Kotani, T. (2019). The role of E2F8 in the human placenta. Molecular medicine reports, 19(1), 293-301.

Morrell, B. C., Zhang, L., Schütz, L. F., Perego, M. C., Maylem, E. R. S., & Spicer, L. J. (2019). Regulation of the transcription factor E2F8 gene expression in bovine ovarian cells. Molecular and cellular endocrinology, 498, 110572.

Chang, H., Song, J., Wu, J., & Zhang, Y. (2018). E2F transcription factor 8 promotes cell proliferation via CCND1/p21 in esophageal squamous cell carcinoma. OncoTargets and therapy, 11, 8165.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-E2F8 Recombinant Antibody (CBFYE-0380) (CAT#: CBMAB-E0686-FY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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