Mouse Anti-EDC3 Recombinant Antibody (E0752) (V2LY-0425-LY1389)

Name: Mouse Anti-EDC3 Recombinant Antibody (E0752)
SKU: V2LY-0425-LY1389
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

E0752

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 209-508 mapping at the C-terminus of human Edc3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Enhancer Of MRNA Decapping 3

Entrez Gene ID

Human	80153
Mouse	353190
Rat	315708

UniProt ID

Human	Q96F86
Mouse	Q8K2D3
Rat	F1M7Z1

Research Area

Binds single-stranded RNA. Involved in the process of mRNA degradation and in the positive regulation of mRNA decapping. May play a role in spermiogenesis and oogenesis.

Biological Process

Deadenylation-independent decapping of nuclear-transcribed mRNA Source: GO_Central
P-body assembly Source: GO_Central

Cellular Location

P-body. Processing bodies (PB).

Involvement in disease

Mental retardation, autosomal recessive 50 (MRT50):
A form of mental retardation, a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior and manifested during the developmental period. MRT50 patients show mild intellectual disability and microcephaly.

Bearss, J. J., Padi, S. K., Singh, N., Cardo‐Vila, M., Song, J. H., Mouneimne, G., ... & Okumura, K. (2021). EDC3 phosphorylation regulates growth and invasion through controlling P‐body formation and dynamics. EMBO reports, 22(4), e50835.

Paquette, D. R., Tibble, R. W., Daifuku, T. S., & Gross, J. D. (2018). Control of mRNA decapping by autoinhibition. Nucleic Acids Research, 46(12), 6318-6329.

Charenton, C., & Graille, M. (2018). mRNA decapping: finding the right structures. Philosophical Transactions of the Royal Society B: Biological Sciences, 373(1762), 20180164.

Mugridge, J. S., Tibble, R. W., Ziemniak, M., Jemielity, J., & Gross, J. D. (2018). Structure of the activated Edc1-Dcp1-Dcp2-Edc3 mRNA decapping complex with substrate analog poised for catalysis. Nature communications, 9(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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